Overview

  • Product name
  • Description
    Goat polyclonal to EXOC7
  • Specificity
    Expected to recognise both isoforms NP_001013861.1 and NP_056034.2.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Zebrafish
  • Immunogen

    Synthetic peptide:

    C-DNIKNDPDKEYN

    , corresponding to internal sequence amino acids 430-441 of Human EXOC7 isoform 1 (NP_001013861.1) and 399-410 of Human EXOC7 isoform 2 (NP_056034.2)

  • Positive control
    • WB: Human lung lysate; IHC-P: Human Adrenal gland tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/16000.
    IHC-P: Use at a concentration of 5 - 10 µg/ml.
    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 74 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • Anti-EXOC7 antibody (ab77352) at 0.5 µg/ml + Human lung lysate in RIPA buffer at 35 µg

      Predicted band size : 74 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human adrenal gland tissue, using ab77352 at 5µg/ml. Note the staining of cytoplasm membranes and cytoplasm of secretory cells in the medulla.

    References

    ab77352 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab77352.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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