Overview

  • Product nameExodus 2 Mouse ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    31.25 pg/ml - 1000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.88 86% - 107%
    Serum 95.51 84% - 105%
    Plasma 94.29 83% - 102%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Exodus 2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Exodus 2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse Exodus 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Exodus 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Exodus 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Exodus 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • RelevanceExodus 2 is one of several CC cytokine genes clustered on the p arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterised by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).
  • Alternative names
    • 6Ckine
    • Beta chemokine exodus 2
    • C-C motif chemokine 21
    • Chemokine (C-C motif) ligand 21
    • CKb9
    • ECL
    • Efficient Chemoattractant for Lymphocytes
    • Exodus2
    • MGC34555
    • OTTHUMP00000000526
    • OTTHUMP00000000527
    • OTTHUMP00000021303
    • SCYA 21
    • SCYA21
    • Secondary lymphoid tissue chemokine
    • SLC
    • Small inducible cytokine A21
    • Small inducible cytokine subfamily A (Cys-Cys), member 21
    • TCA 4
    • TCA4
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

Exodus 2 Mouse ELISA Kit images

  • Representative Standard Curve using ab100667.

  • Representative Standard Curve using ab100667.

Protocols

References for Exodus 2 Mouse ELISA Kit (ab100667)

ab100667 has not yet been referenced specifically in any publications.

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