Overview

  • Product name
    Anti-Exonuclease 1 antibody
    See all Exonuclease 1 primary antibodies
  • Description
    Goat polyclonal to Exonuclease 1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from Human Exonuclease 1.

  • Positive control
    • Human Adrenal and Skeletal muscle tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 94 kDa.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
  • Tissue specificity
    Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
  • Sequence similarities
    Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.
  • Developmental stage
    Highly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein.
  • Cellular localization
    Nucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase.
  • Information by UniProt
  • Database links
  • Alternative names
    • exo1 antibody
    • EXO1_HUMAN antibody
    • ExoI antibody
    • Exonuclease 1 antibody
    • Exonuclease I antibody
    • Exonuclease1 antibody
    • HEX1 antibody
    • hExo I antibody
    • hExo1 antibody
    • hExoI antibody
    • Rad2 nuclease family member homolog of S. cerevisiae exonuclease 1 antibody
    see all

Images

  • ab111017, at 3.75µg/ml, staining Exonuclease 1 in Formalin-fixed, Paraffin-embedded Human Adrenal tissue by Immunohistochemistry.
  • ab111017, at 3.75µg/ml, staining Exonuclease 1 in Formalin-fixed, Paraffin-embedded Human Skeletal Muscle tissue by Immunohistochemistry.

References

ab111017 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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