Overview

  • Product name
    Anti-Exonuclease 1 antibody
    See all Exonuclease 1 primary antibodies
  • Description
    Rabbit polyclonal to Exonuclease 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 474-753 of Human Exonuclease 1 (Uniprot ID: Q9UQ84).

  • Positive control
    • Jurkat and Mouse thymus whole cell lysates; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 94 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
  • Tissue specificity
    Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
  • Sequence similarities
    Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.
  • Developmental stage
    Highly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein.
  • Cellular localization
    Nucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase.
  • Information by UniProt
  • Database links
  • Alternative names
    • exo1 antibody
    • EXO1_HUMAN antibody
    • ExoI antibody
    • Exonuclease 1 antibody
    • Exonuclease I antibody
    • Exonuclease1 antibody
    • HEX1 antibody
    • hExo I antibody
    • hExo1 antibody
    • hExoI antibody
    • Rad2 nuclease family member homolog of S. cerevisiae exonuclease 1 antibody
    see all

Images

  • Anti-Exonuclease 1 antibody (ab155553) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size: 94 kDa



    7.5% SDS PAGE
  • Anti-Exonuclease 1 antibody (ab155553) at 1/1000 dilution + Mouse thymus whole cell lysate at 50 µg

    Predicted band size: 94 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling Exonuclease 1 with ab155553 at 1/500 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).

References

ab155553 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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