Overview

  • Product nameAnti-Exonuclease 1 antibody
    See all Exonuclease 1 primary antibodies
  • Description
    Mouse monoclonal to Exonuclease 1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: ADSLSTTKIK PLGPARASGL SKKPASIQKR KHHNAENKPG LQIKLNELWK NFGFKKDSEK LPPCKKPLSP VRDNIQLTPE AEEDIFNKPE CGRVQRAIFQ , corresponding to amino acids 747-846 of Human Exonuclease 1

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab56472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
    • Tissue specificityHighly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
    • Sequence similaritiesBelongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.
    • Developmental stageHighly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein.
    • Cellular localizationNucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase.
    • Information by UniProt
    • Database links
    • Alternative names
      • exo1 antibody
      • EXO1_HUMAN antibody
      • ExoI antibody
      • Exonuclease 1 antibody
      • Exonuclease I antibody
      • Exonuclease1 antibody
      • HEX1 antibody
      • hExo I antibody
      • hExo1 antibody
      • hExoI antibody
      • Rad2 nuclease family member homolog of S. cerevisiae exonuclease 1 antibody
      see all

    Anti-Exonuclease 1 antibody images



    • Predicted band size : 94 kDa
      Exonuclease 1 antibody (ab56472) at 1ug/lane + A-431 cell lysate at 25ug/lane.

    References for Anti-Exonuclease 1 antibody (ab56472)

    ab56472 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab56472.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"