The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: Antibody detection limit dilution 1/64000.
WB: Use at a concentration of 0.2 - 0.5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 94 kDa).
An additional band of unknown identity was also consistently observed at 38kDa. This band was successfully blocked by incubation with the immunising peptide
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Function5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
Tissue specificityHighly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
Sequence similaritiesBelongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.
Developmental stageHighly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus.
Post-translational modificationsPhosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein.
Cellular localizationNucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase.