• Product nameAnti-Exonuclease 1 antibody
    See all Exonuclease 1 primary antibodies
  • Description
    Goat polyclonal to Exonuclease 1
  • Specificityab77375 is expected to recognise all three reported isoforms (NP_003677.3; NP_006018.3 and NP_569082.1).
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide:


    , corresponding to internal sequence amino acids 372-383 of Human Exonuclease 1 (NP_003677.3; NP_006018.3; NP_569082.1).

  • Positive control
    • HEK293 and A549 cell lysates.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab77375 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab77375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesPeptide ELISA: Antibody detection limit dilution 1/64000.
    WB: Use at a concentration of 0.2 - 0.5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 94 kDa).
    An additional band of unknown identity was also consistently observed at 38kDa. This band was successfully blocked by incubation with the immunising peptide

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
    • Tissue specificityHighly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
    • Sequence similaritiesBelongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.
    • Developmental stageHighly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus.
    • Post-translational
      Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein.
    • Cellular localizationNucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase.
    • Information by UniProt
    • Database links
    • Alternative names
      • exo1 antibody
      • EXO1_HUMAN antibody
      • ExoI antibody
      • Exonuclease 1 antibody
      • Exonuclease I antibody
      • Exonuclease1 antibody
      • HEX1 antibody
      • hExo I antibody
      • hExo1 antibody
      • hExoI antibody
      • Rad2 nuclease family member homolog of S. cerevisiae exonuclease 1 antibody
      see all

    Anti-Exonuclease 1 antibody images

    • Anti-Exonuclease 1 antibody (ab77375) at 0.2 µg/ml + A549 cell lysate (in RIPA buffer) at 35 µg

      Predicted band size : 94 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-Exonuclease 1 antibody (ab77375)

    ab77375 has not yet been referenced specifically in any publications.

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