Anti-Exonuclease 1 antibody (ab95012)
Key features and details
- Rabbit polyclonal to Exonuclease 1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Exonuclease 1 antibody
See all Exonuclease 1 primary antibodies -
Description
Rabbit polyclonal to Exonuclease 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide within Human Exonuclease 1 aa 700-800. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_006018.4
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Positive control
- HeLa and 293T whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab95012 was affinity purified using an epitope specific to Exonuclease 1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 94 kDa.
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IP |
Use at 5-15 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 94 kDa. |
IP
Use at 5-15 µg/mg of lysate. |
Target
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Function
5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis. -
Tissue specificity
Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. -
Sequence similarities
Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. -
Developmental stage
Highly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus. -
Post-translational
modificationsPhosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein. -
Cellular localization
Nucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase. - Information by UniProt
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Database links
- Entrez Gene: 457856 Chimpanzee
- Entrez Gene: 9156 Human
- Omim: 606063 Human
- SwissProt: Q9UQ84 Human
- Unigene: 498248 Human
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Alternative names
- exo1 antibody
- EXO1_HUMAN antibody
- ExoI antibody
see all
Images
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All lanes : Anti-Exonuclease 1 antibody (ab95012) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 94 kDa
Exposure time: 30 seconds -
ab95012 at 1 µg/ml detecting Exonuclease 1 in HeLa whole cell lysate by WB following IP.
Lane 1: ab95012 with 10µg/mg of lysate
Lane 2: IP with an antibody which recognizes an downstream epitope of Exonuclease 1
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab95012 has been referenced in 4 publications.
- Yan S et al. ZGRF1 promotes end resection of DNA homologous recombination via forming complex with BRCA1/EXO1. Cell Death Discov 7:260 (2021). PubMed: 34552057
- Yang G et al. EXO1 Plays a Carcinogenic Role in Hepatocellular Carcinoma and is related to the regulation of FOXP3. J Cancer 11:4917-4932 (2020). PubMed: 32626539
- Xie Y et al. RBX1 prompts degradation of EXO1 to limit the homologous recombination pathway of DNA double-strand break repair in G1 phase. Cell Death Differ 27:1383-1397 (2020). PubMed: 31562368
- Lee KY et al. MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex. Nat Commun 6:7744 (2015). WB . PubMed: 26215093