Anti-Exonuclease 1 antibody - N-terminal (ab82314)

Overview

  • Product nameAnti-Exonuclease 1 antibody - N-terminal
    See all Exonuclease 1 primary antibodies
  • Description
    Rabbit polyclonal to Exonuclease 1 - N-terminal
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide designed within the N-terminal region of Human Exonuclease 1, conjugated to KLH

  • Positive control
    • NCI-H460 cell line lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityAmmonium Sulphate Precipitation
  • Purification notesPrepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 94 kDa.
ELISA 1/1000.

Target

  • Function5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
  • Tissue specificityHighly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
  • Sequence similaritiesBelongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.
  • Developmental stageHighly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein.
  • Cellular localizationNucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase.
  • Information by UniProt
  • Database links
  • Alternative names
    • exo1 antibody
    • EXO1_HUMAN antibody
    • ExoI antibody
    • Exonuclease 1 antibody
    • Exonuclease I antibody
    • Exonuclease1 antibody
    • HEX1 antibody
    • hExo I antibody
    • hExo1 antibody
    • hExoI antibody
    • Rad2 nuclease family member homolog of S. cerevisiae exonuclease 1 antibody
    see all

Anti-Exonuclease 1 antibody - N-terminal images

  • Anti-Exonuclease 1 antibody - N-terminal (ab82314) at 1/50 dilution + NCI-H460 cell line lysates at 35 µg

    Predicted band size : 94 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Exonuclease 1 antibody - N-terminal (ab82314)

ab82314 has not yet been referenced specifically in any publications.

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