Exonuclease 1 overexpression 293T lysate (whole cell) (ab94309)

Overview

  • Product nameExonuclease 1 overexpression 293T lysate (whole cell)
  • Description
    Exonuclease 1 overexpression lysate
  • General notesab94309 is a 293T cell transfected lysate in which Human Exonuclease 1 has been transiently over-expressed using a pCMV-Exonuclease 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundDevelopmental stage: Highly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus. Function: 5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis. PTM: Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein. Similarity: Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. Tissue specificity: Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Exonuclease 1 overexpression 293T lysate (whole cell) images

  • ab94309 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Exonuclease 1 antibody (ab56472) at 1/500 dilution

    Lane 1 : Exonuclease 1 293T Transfected Lysate - Positive Control (ab94309)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution

References for Exonuclease 1 overexpression 293T lysate (whole cell) (ab94309)

ab94309 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"