Overview

  • Product name
  • Description
    Rabbit polyclonal to EXOSC10
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-375 of Human EXOSC10 (UniProt: Q01780).

  • Positive control
    • Raji whole cell lysate; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab154239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 101 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of SKIV2L2, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA.
  • Sequence similarities
    Contains 1 3'-5' exonuclease domain.
    Contains 1 HRDC domain.
  • Cellular localization
    Cytoplasm. Nucleus, nucleolus. Nucleus. Strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form.
  • Information by UniProt
  • Database links
  • Alternative names
    • Autoantigen PM/Scl 2 antibody
    • Exosc10 antibody
    • Exosome component 10 antibody
    • EXOSX_HUMAN antibody
    • P100 polymyositis scleroderma overlap syndrome associated autoantigen antibody
    • P100 polymyositis-scleroderma overlap syndrome-associated autoantigen antibody
    • p2 antibody
    • p3 antibody
    • p4 antibody
    • PM Scl antibody
    • PM/Scl 100 antibody
    • PM/Scl-100 antibody
    • PMSCL antibody
    • PMSCL2 antibody
    • Polymyositis/scleroderma autoantigen 100 kDa antibody
    • Polymyositis/scleroderma autoantigen 2 100 kDa antibody
    • Polymyositis/scleroderma autoantigen 2 antibody
    • RRP6 antibody
    • Rrp6p antibody
    see all

Images

  • Anti-EXOSC10 antibody (ab154239) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 101 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling EXOSC10 with ab154239 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

References

ab154239 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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