Anti-EXOSC10/RRP6 antibody (ab154239)
Key features and details
- Rabbit polyclonal to EXOSC10/RRP6
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EXOSC10/RRP6 antibody
See all EXOSC10/RRP6 primary antibodies -
Description
Rabbit polyclonal to EXOSC10/RRP6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant fragment corresponding to Human EXOSC10/RRP6 aa 1-375.
Database link: Q01780 -
Positive control
- Raji whole cell lysate; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 101 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 101 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of SKIV2L2, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA. -
Sequence similarities
Contains 1 3'-5' exonuclease domain.
Contains 1 HRDC domain. -
Cellular localization
Cytoplasm. Nucleus, nucleolus. Nucleus. Strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. - Information by UniProt
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Database links
- Entrez Gene: 535340 Cow
- Entrez Gene: 5394 Human
- Entrez Gene: 50912 Mouse
- Omim: 605960 Human
- SwissProt: Q01780 Human
- SwissProt: P56960 Mouse
- Unigene: 632368 Human
- Unigene: 266635 Mouse
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Alternative names
- Autoantigen PM/Scl 2 antibody
- Exosc10 antibody
- Exosome component 10 antibody
see all
Images
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Anti-EXOSC10/RRP6 antibody (ab154239) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 101 kDa
7.5% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling EXOSC10/RRP6 with ab154239 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154239 has not yet been referenced specifically in any publications.