The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 23166521
Use a concentration of 1 - 2 µg/ml.
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa). Minor additional bands may be detected in some cell preparations.
FunctionPutative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of SKIV2L2, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA.
Cellular localizationCytoplasm. Nucleus, nucleolus. Nucleus. Strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form.
Lanes 1 - 2 : Anti-EXOSC10 antibody (ab50558) at 1 µg/ml Lane 3 : Anti-EXOSC10 antibody (ab50558) at 2 µg/ml
Lane 1 : HeLa nuclear extract Lane 2 : HeLa nuclear extract with PM/Scl-100 peptide Lane 3 : HeLa nuclear extract
Secondary Goat Anti-Rabbit IgG, Peroxidase conjugate with a chemiluminescent substrate.
Predicted band size : 101 kDa Observed band size : 101 kDa
Immunocytochemistry/ Immunofluorescence - EXOSC10 antibody (ab50558)Image courtesy of Dr Jonathan Houseley by Abreview.
ab50558 staining EXOSC10 in murine NIH3T3 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in 3.7% formaldehyde and permeabilized overnight in 70% ethanol at -20°C and then 30 minutes with 0.5% Triton. Samples were then blocked and incubated with ab50558 at a 1/435 dilution for 30 minutes at 20°C. The secondary used was a goat anti-rabbit polyclonal conjugated to Alex Fluor 594, used at a 1/1000 dilution. Nuclei stained with DAPI.
Hett A & West S Inhibition of U4 snRNA in Human Cells Causes the Stable Retention of Polyadenylated Pre-mRNA in the Nucleus. PLoS One9:e96174 (2014).
Read more (PubMed: 24796696) »
Zonta E et al. The RNA helicase DDX5/p68 is a key factor promoting c-fos expression at different levels from transcription to mRNA export. Nucleic Acids Res41:554-64 (2013).
Read more (PubMed: 23143267) »