The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/50 - 1/70.
EXOSC3 is a component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions. It is required for the 3'processing of the 7S pre-RNA to the mature 5.8S rRNA. Has a 3'-5' exonuclease activity. [Swiss-Prot]
Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling EXOSC3 with ab184961 at 1/100 dilution (red) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
Western blot - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)
All lanes : Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961) at 1/10000 dilution
Lane 1 : 293T cell lysates Lane 2 : HeLa cell lysates Lane 3 : Jurkat cell lysates Lane 4 : Raji cell lysates
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunoprecipitation analysis of 293T lysates (Lane 1) labeling EXOSC3 with ab184961 at a 1/50 dilution and a negative control PBS (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at a 1500 dilution.
Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling EXOSC3 with ab184961 at 1/50 dilution. Counterstained with Hematoxylin.