Overview

  • Product name
  • Description
    Rabbit polyclonal to EXOSC4
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide: AGLELLSDQG YRVDGRRAGE LRKIQARMGV FAQADGSAYI EQGNTKALAV, corresponding to amino acids 2-51 of Human EXOSC4

  • Positive control
    • Jurkat cell lysate. Human heart tissue. Human liver tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/62500.

WB Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Relevance
    EXOSC4 belongs to the RNase PH family. It is a component of the exosome 3'->5' exoribonuclease complex and is required for the 3' processing of the 7S pre-RNA to the mature 5.8S rRNA. It has a 3'-5' exonuclease activity.
  • Cellular localization
    Cytoplasm. Nucleus, nucleolus.
  • Database links
  • Alternative names
    • EXOSC 4 antibody
    • Exosome complex component RRP41 antibody
    • Exosome complex exonuclease RRP41 antibody
    • Exosome component 4 antibody
    • Exosome component Rrp41 antibody
    • FLJ20591 antibody
    • hRrp41p antibody
    • p12A antibody
    • Ribosomal RNA processing protein 41 antibody
    • RRP 41 antibody
    • RRP41 antibody
    • RRP41A antibody
    • Rrp41p antibody
    • SKI 6 antibody
    • SKI6 antibody
    • Ski6p antibody
    see all

Images

  • All lanes : Anti-EXOSC4 antibody (ab66672) at 2.5 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
    Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling EXOSC4 with ab66672 at 4-8µg/ml. Arrow indicate positively stained hepatocyte. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling EXOSC4 with ab66672 at 4-8µg/ml. Arrow indicate positively labelled myocardial cells. Magnification: 400X.

References

ab66672 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab66672.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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