Overview

  • Product nameAnti-EXOSC4 antibody
    See all EXOSC4 primary antibodies
  • Description
    Mouse monoclonal to EXOSC4
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment: MAGLELLSDQ GYRVDGRRAG ELRKIQARMG VFAQADGSAY IEQGNTKALA VVYGPHEIRG SRARALPDRA LVNCQYSSAT FSTGERKRRP HGDRKSCEMG, corresponding to amino acids 1-100 of Human EXOSC4 (NP_061910) with a proprietory tag.

  • Positive control
    • EXOSC4 transfected 293T cell lysate. Recombinant tagged human EXOSC4 fragment.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 26 kDa.
ELISA Use at an assay dependent dilution.

Target

  • RelevanceEXOSC4 belongs to the RNase PH family. It is a component of the exosome 3'->5' exoribonuclease complex and is required for the 3' processing of the 7S pre-RNA to the mature 5.8S rRNA. It has a 3'-5' exonuclease activity.
  • Cellular localizationCytoplasm. Nucleus, nucleolus.
  • Database links
  • Alternative names
    • EXOSC 4 antibody
    • Exosome complex component RRP41 antibody
    • Exosome complex exonuclease RRP41 antibody
    • Exosome component 4 antibody
    • Exosome component Rrp41 antibody
    • FLJ20591 antibody
    • hRrp41p antibody
    • p12A antibody
    • Ribosomal RNA processing protein 41 antibody
    • RRP 41 antibody
    • RRP41 antibody
    • RRP41A antibody
    • Rrp41p antibody
    • SKI 6 antibody
    • SKI6 antibody
    • Ski6p antibody
    see all

Anti-EXOSC4 antibody images

  • Anti-EXOSC4 antibody (ab77458) at 1 µg/ml + Recombinant tagged human EXOSC4 fragment at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 26 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-EXOSC4 antibody (ab77458) at 1 µg/ml

    Lane 1 : EXOSC4 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 26 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)

References for Anti-EXOSC4 antibody (ab77458)

ab77458 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"