Overview

  • Product nameAnti-EXOSC7 antibody
    See all EXOSC7 primary antibodies
  • Description
    Rabbit polyclonal to EXOSC7
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog
  • Immunogen

    A region within synthetic peptide: KPNEGYLEFF VDCSASATPE FEGRGGDDLG TEIANTLYRI FNNKSSVDLK, corresponding to N terminal amino acids 73-122 of Human EXOSC7

  • Positive control
    • Jurkat cell lysate; human kidney tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

Target

  • FunctionNon-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes.
  • Sequence similaritiesBelongs to the RNase PH family.
  • Cellular localizationNucleus > nucleolus. Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • EAP1 antibody
    • EXOS7_HUMAN antibody
    • Exosc7 antibody
    • Exosome complex component RRP42 antibody
    • Exosome complex exonuclease RRP42 antibody
    • Exosome component 7 antibody
    • FLJ26543 antibody
    • hRrp42p antibody
    • KIAA0116 antibody
    • p8 antibody
    • Ribosomal RNA processing protein 42 antibody
    • Ribosomal RNA-processing protein 42 antibody
    • RRP42 antibody
    • Rrp42p antibody
    see all

Anti-EXOSC7 antibody images

  • Lane 1 : Marker
    Lane 2 : Anti-EXOSC7 antibody (ab50902) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 32 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical staining of paraffin-embedded human kidney tissue, positively staining epithelial cells of the renal tubule (indicated by arrows), using ab50902 at a concentration of 4 µg/ml. Magnification 400X.

References for Anti-EXOSC7 antibody (ab50902)

ab50902 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"