Overview

  • Product nameAnti-EXOSC7 antibody
    See all EXOSC7 primary antibodies
  • Description
    Mouse polyclonal to EXOSC7
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1 - 291 of Human EXOSC7 (NP_055819.1)

  • Positive control
    • WB: Jurkat cell lysate; ICC/IF: HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab89104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionNon-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes.
  • Sequence similaritiesBelongs to the RNase PH family.
  • Cellular localizationNucleus > nucleolus. Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • EAP1 antibody
    • EXOS7_HUMAN antibody
    • Exosc7 antibody
    • Exosome complex component RRP42 antibody
    • Exosome complex exonuclease RRP42 antibody
    • Exosome component 7 antibody
    • FLJ26543 antibody
    • hRrp42p antibody
    • KIAA0116 antibody
    • p8 antibody
    • Ribosomal RNA processing protein 42 antibody
    • Ribosomal RNA-processing protein 42 antibody
    • RRP42 antibody
    • Rrp42p antibody
    see all

Anti-EXOSC7 antibody images

  • Anti-EXOSC7 antibody (ab89104) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size : 32 kDa
  • All lanes : Anti-EXOSC7 antibody (ab89104) at 1 µg/ml

    Lane 1 : EXOSC7-transfected 293T cell lysate
    Lane 2 : non transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • Analysis of EXOSC7 expression in PFA-fixed, permeabilized HeLa cells using ab89104 at 10 µg/ml, followed by a fluorescein-conjugated secondary antibody.

References for Anti-EXOSC7 antibody (ab89104)

ab89104 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"