Overview

  • Product name
  • Description
    Rabbit polyclonal to EXOSC8
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the C terminal region of human EXOSC8.

  • Positive control
    • HL60 cell line lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 30 kDa.
ELISA 1/1000.

Target

  • Function
    Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC8 binds to ARE-containing RNAs.
  • Sequence similarities
    Belongs to the RNase PH family.
  • Cellular localization
    Cytoplasm. Nucleus. Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA421P11.3 antibody
    • CBP interacting protein 3 antibody
    • CIP3 antibody
    • EAP2 antibody
    • EC 3.1.13 antibody
    • EXOS8_HUMAN antibody
    • EXOSC8 antibody
    • Exosome complex component RRP43 antibody
    • Exosome complex exonuclease RRP43 antibody
    • Exosome component 8 antibody
    • OIP-2 antibody
    • OIP2 antibody
    • Opa interacting protein 2 antibody
    • Opa-interacting protein 2 antibody
    • OTTHUMP00000042274 antibody
    • p9 antibody
    • Ribosomal RNA processing protein 43 antibody
    • Ribosomal RNA processing protein 43, S. cerevisiae, homolog of antibody
    • Ribosomal RNA-processing protein 43 antibody
    • RP11 421P11.3 antibody
    • RRP43 antibody
    • Rrp43p antibody
    see all

Anti-EXOSC8 antibody images

  • Anti-EXOSC8 antibody (ab82290) at 1/50 dilution + HL60 cell line lysates at 35 µg

    Predicted band size : 30 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Immunofluorescence analysis of Human skin fibroblasts, staining EXOSC8 with ab82290.

    Cells were fixed with paraformaldehyde, permeabilized with 0.1 % Triton-X-100 and blocked with 5% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 5% serum) for 3 hours at 25°C. An Cy2®-conjugated donkey anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

    See Abreview

References for Anti-EXOSC8 antibody (ab82290)

ab82290 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application
Western blot
Sample
Human Cell lysate - other (Human skin fibroblast)
Loading amount
40 µg
Specification
Human skin fibroblast
Gel Running Conditions
Reduced Denaturing (12.5%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 21 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Skin Fibroblasts)
Specification
Skin Fibroblasts
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 13 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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