Recombinant
RabMAb

Anti-Exportin 4 antibody [EPR4442(2)] (ab133237)

Overview

  • Product name
    Anti-Exportin 4 antibody [EPR4442(2)]
    See all Exportin 4 primary antibodies
  • Description
    Rabbit monoclonal [EPR4442(2)] to Exportin 4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminus of Human Exportin 4 (UniProtKB/TrEMBL Q0VG75).

  • Positive control
    • Jurkat, HeLa, SW480, C6, Raw264.7 and PC12 cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 127 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Mediates the nuclear export of proteins (cargos) with broad substrate specificity. In the nucleus binds cooperatively to its cargo and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. XPO4 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
    • Sequence similarities
      Belongs to the exportin family.
    • Cellular localization
      Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Exp4 antibody
      • Exportin-4 antibody
      • exportin4 antibody
      • FLJ13046 antibody
      • KIAA1721 antibody
      • xpo4 antibody
      • XPO4_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Exportin 4 antibody [EPR4442(2)] (ab133237) at 1/1000 dilution

      Lane 1 : Jurkat cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : SW480 cell lysates
      Lane 4 : C6 cell lysates
      Lane 5 : Raw264.7 cell lysates
      Lane 6 : PC12 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 127 kDa

    References

    ab133237 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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