Overview

  • Product nameAnti-Exportin-5 antibody
    See all Exportin-5 primary antibodies
  • Description
    Rabbit polyclonal to Exportin-5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 190-239 (LNFLLSTLQE NVNKYQQMKT DASQEAEAQA NCRVSIAALN TLAGYIDWVS) of Rat Exportin-5 (NP_001102259).

  • Positive control
    • Rat liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab116098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 137 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP-and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi).
    Mediates the nuclear export of micro-RNA precursors, which form short hairpins. Also mediates the nuclear export of synthetic short hairpin RNAs used for RNA interference, and adenovirus VA1 dsRNA. In some circumstances can also mediate the nuclear export of deacylated and aminoacylated tRNAs. Specifically recognizes dsRNAs that lack a 5'-overhang in a sequence-independent manner, have only a short 3'-overhang, and that have a double-stranded length of at least 15 base-pairs. Binding is dependent on Ran-GTP.
  • Tissue specificityExpressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas.
  • Sequence similaritiesBelongs to the exportin family.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Exp 5 antibody
    • Exp5 antibody
    • Exportin 5 antibody
    • Exportin-5 antibody
    • Exportin5 antibody
    • FLJ14239 antibody
    • FLJ32057 antibody
    • FLJ45606 antibody
    • KIAA1291 antibody
    • OTTHUMP00000016472 antibody
    • Ran binding protein 21 antibody
    • Ran-binding protein 21 antibody
    • Ranbp 21 antibody
    • Ranbp21 antibody
    • Xpo 5 antibody
    • xpo5 antibody
    • XPO5_HUMAN antibody
    see all

Anti-Exportin-5 antibody images

  • Anti-Exportin-5 antibody (ab116098) at 1 µg/ml + Rat liver lysate at 10 µg

    Predicted band size : 137 kDa

References for Anti-Exportin-5 antibody (ab116098)

ab116098 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab116098.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"