Overview

  • Product name
    Anti-Exportin T antibody
  • Description
    Rabbit polyclonal to Exportin T
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from the N-terminal region of Human Exportin T (NP_009166), conjugated to KLH

  • Positive control
    • Ramos cell line lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab85365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 110 kDa.
IHC-P 1/50 - 1/100.

Target

  • Relevance
    This gene encodes a protein belonging to the RAN GTPase exportin family that mediates export of tRNA from the nucleus to the cytoplasm. Translocation of tRNA to the cytoplasm occurs once exportin has bound both tRNA and GTP bound RAN.
  • Cellular localization
    Nuclear; once bound to tRNA and Ran the complex translocates to the cytoplasm. Shuttles between the nucleus and the cytoplasm.
  • Database links
  • Alternative names
    • 1110004L07Rik antibody
    • 3110065H13Rik antibody
    • AI452076 antibody
    • C79645 antibody
    • Exportin tRNA antibody
    • Exportin(tRNA) antibody
    • Nuclear export receptor for tRNAs antibody
    • tRNA exportin antibody
    • XPO3 antibody
    • XPOT antibody
    see all

Anti-Exportin T antibody images

  • Anti-Exportin T antibody (ab85365) at 1/100 dilution + Human brain tissue lysate at 50 µg

    Secondary
    HRP-conjugated Goat anti-rabbit IgG at 1/8000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 110 kDa
    Observed band size : 110 kDa


    Exposure time : 3 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Anti-Exportin T antibody (ab85365) at 1/50 dilution + Ramos cell line lysates at 35 µg

    Predicted band size : 110 kDa
    Observed band size : 110 kDa
    Additional bands at : <55 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical staining of formalin-fixed and paraffin-embedded human lymph by ab85365 followed by staining with a HRP-conjugated secondary antibody and DAB staining.

References for Anti-Exportin T antibody (ab85365)

ab85365 has not yet been referenced specifically in any publications.

Product Wall

Application
ELISA
Sample
Human Tissue sections (BRAIN)
Specification
BRAIN
Type
Indirect
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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Submitted Dec 06 2013

Application
Immunoprecipitation
Immuno-precipitation step
Other - Dynabead IgG
Sample
Human Tissue lysate - whole (BRAIN)
Specification
BRAIN
Total protein in input
500 µg
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Submitted Dec 06 2013

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Human Tissue lysate - whole (brain)
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

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Verified customer

Submitted Dec 03 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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