EXPOSE Mouse and Rabbit Specific AP (red) Detection IHC Kit (ab94734)

Overview

  • Product name
    EXPOSE Mouse and Rabbit Specific AP (red) Detection IHC Kit
    See all Alkaline phosphatase conjugate kits
  • Detection method
    Colorimetric
  • Assay type
    Micro-Polymer
  • Product overview

    This detection kit offers a red alternative to the brown of the DAB product for antigen detection. This technique involves the sequential incubation of the specimen with an unconjugated mouse or rabbit primary antibody specific to the target antigen, a secondary antibody-AP conjugate which reacts with the primary antibody, and AP-compatible chromogen (Fast Red), so that a detectable signal is generated from converting Naphthol Phosphate substrate to a red colored product at the site where the antibody portion of the conjugate is bound to its target.


    Reacts with Mouse and Rabbit primary antibodies.

  • Tested applications
    Suitable for: IHC-Pmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab94734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

Images

  • Immunohistochemical analysis of Human Tonsil tissue labeling Ki-67 using the ab94735 anti-EXPOSE Mouse and Rabbit Specific AP (red) Detection IHC Kit.

References

This product has been referenced in:
  • Pópulo H  et al. SDHD promoter mutations are rare events in cutaneous melanomas but SDHD protein expression is downregulated in advanced cutaneous melanoma. PLoS One 12:e0180392 (2017). IHC ; Human . Read more (PubMed: 28662141) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting Abcam regarding ab94734.


I have confirmed that this kit contains a cocktail of goat anti-rabbit IG and goat anti-mouse IgG antibodies so yes, this product will recognize all primary IgG antibodies of murine and ra...

Read More

Thank you for your enquiry. 1) The Magnesium Chloride solution is actually the Co-factor (Enhancer) component. Please rest assured that you have received all the components in the kit. 2) Please mix 100ul of Naphthol Phosphate with 100ul of Fast...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up