EXPOSE Mouse and Rabbit Specific AP (red) Detection IHC Kit (ab94734)

Overview

  • Product nameEXPOSE Mouse and Rabbit Specific AP (red) Detection IHC Kit
    See all Alkaline phosphatase conjugate kits
  • Detection methodColorimetric
  • Assay typeMicro-Polymer
  • Product overview

    This detection kit offers a red alternative to the brown of the DAB product for antigen detection. This technique involves the sequential incubation of the specimen with an unconjugated mouse or rabbit primary antibody specific to the target antigen, a secondary antibody-AP conjugate which reacts with the primary antibody, and AP-compatible chromogen (Fast Red), so that a detectable signal is generated from converting Naphthol Phosphate substrate to a red colored product at the site where the antibody portion of the conjugate is bound to its target.

     

    Reacts with Mouse and Rabbit primary antibodies.

  • Tested applicationsSuitable for: IHC-Pmore details

Properties

  • RelevanceAlkaline phosphatase (AP) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. Alkaline phosphatase conjugate is used in detection systems for immunohistochemistry techniques.
  • Alternative names
    • AP conjugate

Applications

Our Abpromise guarantee covers the use of ab94734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

EXPOSE Mouse and Rabbit Specific AP (red) Detection IHC Kit images

  • Immunohistochemical analysis of Human Tonsil tissue labeling Ki-67 using the ab94735 anti-EXPOSE Mouse and Rabbit Specific AP (red) Detection IHC Kit.

References for EXPOSE Mouse and Rabbit Specific AP (red) Detection IHC Kit (ab94734)

ab94734 has not yet been referenced specifically in any publications.

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Thank you for contacting Abcam regarding ab94734.


I have confirmed that this kit contains a cocktail of goat anti-rabbit IG and goat anti-mouse IgG antibodies so yes, this product will recognize all primary IgG antibodies of murine and ra...

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Thank you for your enquiry. 1) The Magnesium Chloride solution is actually the Co-factor (Enhancer) component. Please rest assured that you have received all the components in the kit. 2) Please mix 100ul of Naphthol Phosphate with 100ul of Fast...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"