This detection kit offers a red alternative to the brown of the DAB product for antigen detection. This technique involves the sequential incubation of the specimen with an unconjugated mouse or rabbit primary antibody specific to the target antigen, a secondary antibody-AP conjugate which reacts with the primary antibody, and AP-compatible chromogen (Fast Red), so that a detectable signal is generated from converting Naphthol Phosphate substrate to a red colored product at the site where the antibody portion of the conjugate is bound to its target.
Reacts with Mouse and Rabbit primary antibodies.
|Components||15 ml||60 ml||125 ml|
|AP Conjugate polyvalent||1 x 15ml||1 x 60ml||1 x 125ml|
|Co-factor (Enhancer)||1 x 15ml||1 x 60ml||1 x 125ml|
|Fast Red Chromogen||1 x 15ml||1 x 60ml||1 x 125ml|
|Napthol Phosphate||1 x 15ml||1 x 60ml||1 x 125ml|
|Protein block||1 x 15ml||1 x 60ml||1 x 125ml|
Our Abpromise guarantee covers the use of ab94734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
Immunohistochemical analysis of Human Tonsil tissue labeling Ki-67 using the ab94735 anti-EXPOSE Mouse and Rabbit Specific AP (red) Detection IHC Kit.
ab94734 has not yet been referenced specifically in any publications.