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The reagents in this kit constitute a biotin-free immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a secondary antibody-HRP conjugate which reacts with the primary antibody, and substrate-chromogen (AEC).
|Components||15 ml||60 ml||125 ml|
|AEC Single Solution||1 x 15ml||1 x 60ml||1 x 125ml|
|Goat anti-rabbit HRP Conjugate||1 x 15ml||1 x 30ml||1 x 125ml|
|Hydrogen Peroxide Block||1 x 15ml||1 x 60ml||1 x 125ml|
|Mouse Specifying Reagent (Complement)||1 x 15ml||1 x 60ml||1 x 125ml|
|Protein block||1 x 15ml||1 x 60ml||1 x 125ml|
Our Abpromise guarantee covers the use of ab93686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
Immunohistochemical staining of p53 in human colon adenocarcinoma with anti-p53 antibody at a dilution of 1/400 and detected using ab93686.
Immunohistochemical staining of smooth muscle actin in human small intestine with ab7817 at a dilution of 1/200 and detected using ab93686.