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The reagents in this kit constitute a biotin-free immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a secondary antibody-HRP conjugate which reacts with the primary antibody, and substrate-chromogen (DAB).
This kit is suitable for staining animal tissues, except mouse and rabbit tissues. The polyvalent-HRP in the kit contains anti-mouse and anti-rabbit secondary antibodies (conjugated with HRP), so the secondary Ab-HRP will non-specifically bind to mouse and rabbit tissues.
DAB is a suspected carcinogen. Handle with care.
Contains hydrogen peroxide.
|Components||15 ml||60 ml||125 ml|
|50x DAB Chromogen||1 x 0.5ml||1 x 2ml||1 x 4ml|
|DAB substrate||1 x 15ml||1 x 60ml||1 x 125ml|
|Goat anti-rabbit HRP Conjugate||1 x 15ml||1 x 60ml||1 x 125ml|
|Hydrogen Peroxide Block||1 x 15ml||1 x 60ml||1 x 125ml|
|Mouse Specifying Reagent (Complement)||1 x 15ml||1 x 60ml||1 x 125ml|
|Protein block||1 x 15ml||1 x 60ml||1 x 125ml|
Our Abpromise guarantee covers the use of ab80436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|