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This detection kit offers a red alternative to the brown of the DAB product for antigen detection. This technique involves the sequential incubation of the specimen with an unconjugated rabbit primary antibody specific to the target antigen, a secondary antibody- AP conjugate which reacts with the primary antibody, and AP-compatible chromogen (Fast Red), so that a detectable signal is generated from converting Naphthol Phosphate substrate to a red colored product at the site where the antibody portion of the conjugate is bound to its target.
Handle Enhancer, Naphthol Phosphate and Fast Red Chromogen with care.
|Components||15 ml||60 ml||125 ml|
|AP Conjugate GRI||1 x 15ml||1 x 60ml||1 x 125ml|
|Co-factor (Enhancer)||1 x 15ml||1 x 60ml||1 x 125ml|
|Fast Red Chromogen||1 x 15ml||1 x 60ml||1 x 125ml|
|Napthol Phosphate||1 x 15ml||1 x 60ml||1 x 125ml|
|Protein block||1 x 15ml||1 x 60ml||1 x 125ml|
Our Abpromise guarantee covers the use of ab94737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
ab94737 has not yet been referenced specifically in any publications.