EXPOSE Rabbit Specific AP (red) Detection IHC Kit (ab94737)

Overview

  • Product nameEXPOSE Rabbit Specific AP (red) Detection IHC Kit
    See all Alkaline phosphatase conjugate kits
  • Detection methodColorimetric
  • Assay typeMicro-Polymer
  • Product overview

    This detection kit offers a red alternative to the brown of the DAB product for antigen detection. This technique involves the sequential incubation of the specimen with an unconjugated rabbit primary antibody specific to the target antigen, a secondary antibody- AP conjugate which reacts with the primary antibody, and AP-compatible chromogen (Fast Red), so that a detectable signal is generated from converting Naphthol Phosphate substrate to a red colored product at the site where the antibody portion of the conjugate is bound to its target.

  • Notes

    Handle Enhancer, Naphthol Phosphate and Fast Red Chromogen with care.

  • Tested applicationsSuitable for: IHC-Pmore details

Properties

  • RelevanceAlkaline phosphatase (AP) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. Alkaline phosphatase conjugate is used in detection systems for immunohistochemistry techniques.
  • Alternative names
    • AP conjugate

Applications

Our Abpromise guarantee covers the use of ab94737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

References for EXPOSE Rabbit Specific AP (red) Detection IHC Kit (ab94737)

ab94737 has not yet been referenced specifically in any publications.

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I can confirm that The AP conjugate GRI reagent is a Goat anti-rabbit antibody which is conjugated to Alkaline Phosphatase.

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The conjugate in ab94737 kit is a Goat anti-rabbit AP conjugate.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"