EXPOSE Rabbit specific HRP-AEC detection IHC kit (ab94361)

Overview

  • Product nameEXPOSE Rabbit specific HRP-AEC detection IHC kit
    See all HRP/AEC Detection Kit kits
  • Detection methodColorimetric
  • Assay typeMicro-Polymer
  • Product overview

    The reagents in this kit constitute a biotin-free immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a rabbit specific HRP conjugate which reacts with the primary antibody, and substrate-chromogen (AEC).

  • Tested applicationsSuitable for: IHC-Pmore details

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 15 ml 60 ml 125 ml
    AEC Single Solution 1 x 15ml 1 x 60ml 1 x 125ml
    Goat anti-rabbit HRP Conjugate 1 x 15ml 1 x 60ml 1 x 125ml
    Hydrogen Peroxide Block 1 x 15ml 1 x 60ml 1 x 125ml
    Protein block 1 x 15ml 1 x 60ml 1 x 125ml
  • Research areas
  • RelevanceAn HRP/AEC Detection Kit is a labeled immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate.
  • Alternative names
    • carbazole
    • Horseradish Peroxidase

Associated products

Applications

Our Abpromise guarantee covers the use of ab94361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

EXPOSE Rabbit specific HRP-AEC detection IHC kit images

  • Immunohistochemical analysis of Human Tonsil tissue labeling Ki-67 using the ab94361 anti-EXPOSE Rabbit specific HRP-AEC detection IHC kit.

References for EXPOSE Rabbit specific HRP-AEC detection IHC kit (ab94361)

This product has been referenced in:
  • Hsu PL  et al. Extracellular matrix protein CCN1 regulates cardiomyocyte apoptosis in mice with stress-induced cardiac injury. Cardiovasc Res 98:64-72 (2013). Read more (PubMed: 23329650) »

See 1 Publication for this product

Product Wall

IHC-P on mice tibia for DMP1 and Periostin

Excellent Excellent 5/5 (Ease of Use)
Abreviews
EXPOSE kit was used for IHC on paraffin sections of mice tibia. I immunolabeled DMP1 and Periostin. I observed very little background in the bone marrow, compare to Streptavidin HRP method, and almost zero background in the bone matrix. Also, this kit can be useful for double IHC in combination with Streptavidin AP (pic. DMP1 & Sclerostin).
Username

Mr. Vasily Gnyubkin

Verified customer

Submitted Jan 29 2014

Thank you for replying with that information.

As I mentioned over the phone, I have spoken to the source of these kits and we do not have any experimental data using either the ACE or DABdetection kits (ab64260, ab64261, ab80437 and ab94361)f...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"