The reagents in this kit constitute a biotin-free immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a rabbit specific HRP conjugate which reacts with the primary antibody, and substrate-chromogen (DAB).
DAB is a suspected carcinogen. Handle with care.
Contains hydrogen peroxide.
|Components||15 ml||60 ml||125 ml|
|50x DAB Chromogen||1 x 1ml||1 x 2ml||1 x 4ml|
|DAB substrate||1 x 15ml||1 x 60ml||1 x 125ml|
|Goat anti-rabbit HRP Conjugate||1 x 15ml||1 x 60ml||1 x 125ml|
|Hydrogen Peroxide Block||1 x 15ml||1 x 60ml||1 x 125ml|
|Protein block||1 x 15ml||1 x 60ml||1 x 125ml|
Our Abpromise guarantee covers the use of ab80437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
ab18723 staining 6 week rat brain tissue subventricular zone (SVZ) by IHC-P using rabbit-specific EXPOSE IHC detection kit (ab80437). Formalin fixed paraffin embedded tissue sections were pre-treated using heat mediated antigen retrieval (using a pressure cooker) with sodium citrate buffer (pH6) for 30 mins. The section was incubated with ab18723, 0.1µg/ml, for 1 hour at room temperature. DAB was used as the chromogen and the section was counterstained with haematoxylin and mounted with DPX.