Overview

  • Product nameEXPOSE Rabbit specific HRP/DAB detection IHC kit
    See all HRP/DAB detection kit kits
  • Detection methodColorimetric
  • Assay typeMicro-Polymer
  • Product overview

    The reagents in this kit constitute a biotin-free immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a rabbit specific HRP conjugate which reacts with the primary antibody, and substrate-chromogen (DAB).

  • Notes

    DAB is a suspected carcinogen. Handle with care.

    Contains hydrogen peroxide.

  • Tested applicationsSuitable for: IHC-Pmore details

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 15 ml 60 ml 125 ml
    50x DAB Chromogen 1 x 1ml 1 x 2ml 1 x 4ml
    DAB substrate 1 x 15ml 1 x 60ml 1 x 125ml
    Goat anti-rabbit HRP Conjugate 1 x 15ml 1 x 60ml 1 x 125ml
    Hydrogen Peroxide Block 1 x 15ml 1 x 60ml 1 x 125ml
    Protein block 1 x 15ml 1 x 60ml 1 x 125ml
  • Research areas
  • RelevanceHRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.

Applications

Our Abpromise guarantee covers the use of ab80437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

EXPOSE Rabbit specific HRP/DAB detection IHC kit images

  • ab18723 staining 6 week rat brain tissue subventricular zone (SVZ) by IHC-P using rabbit-specific EXPOSE IHC detection kit (ab80437). Formalin fixed paraffin embedded tissue sections were pre-treated using heat mediated antigen retrieval (using a pressure cooker) with sodium citrate buffer (pH6) for 30 mins. The section was incubated with ab18723, 0.1µg/ml, for 1 hour at room temperature. DAB was used as the chromogen and the section was counterstained with haematoxylin and mounted with DPX.

References for EXPOSE Rabbit specific HRP/DAB detection IHC kit (ab80437)

This product has been referenced in:
  • Honková L  et al. Epithelial basement membrane thickening is related to TGF-Beta 1 expression in children with chronic respiratory diseases. Pediatr Allergy Immunol 25:593-9 (2014). Read more (PubMed: 25201521) »
  • Vreeland AC  et al. Cellular retinoic acid-binding protein 2 inhibits tumor growth by two distinct mechanisms. J Biol Chem 289:34065-73 (2014). IHC-P ; Mouse . Read more (PubMed: 25320093) »

See all 3 Publications for this product

Product Wall

ab64226 is the protein block used in ab80437. A link to ab64226 is below:

The Goat anti-rabbit HRP conjugate in ab80437 is pre-adsorbed against mouse IgG, so it should not cross react with mouse tissue.

I have contacted the lab who have agreed to add the blocking peptide for ab37542 to the catalogue. The product is called BAIAP2 peptide, ab170411.

As for EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC Kit, I can confirm that it does h...

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Thank you for contacting us.

The number of slides that could be used with this kit depends on the tissue sections, and the volume of reagents per slide. The volume used should be enough to cover them. We normally use 200ul per slide in house,...

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Thank you for your email.

I am happy to hear that you have been able to reduce the background. It is unfortunate, that the specific staining is not visible.

I am happy to help to troubleshoot, however it is difficult for me with the...

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Thank you for your phone call yesterday.

The Protein Block in the kit does not contain any rabbit serum. The laboratory hasnot tested this kit with the addition of a rabbit serum blocking step. However,in theory if the source of the backgroun...

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Thank you for replying with that information.

As I mentioned over the phone, I have spoken to the source of these kits and we do not have any experimental data using either the ACE or DABdetection kits (ab64260, ab64261, ab80437 and ab94361)f...

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Thank you for your reply.

Abreviews for ab32042 Anti-active Caspase 3 antibody [E83-77] and ab59348 Anti-Bcl2 antibody can be submitted via the website. Submitting your protocol and data using our Abreview system will take about 10 minutes. ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for contacting us. The EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC kit (ab80436) can be used to detect mouse and rabbit primary antibodies. You are therefore correct in thinking that the kit would need to be modified in order t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"