Recombinant
RabMAb

Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)

Overview

  • Product name
    Anti-Extracellular matrix protein 1 antibody [EPR6701]
    See all Extracellular matrix protein 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6701] to Extracellular matrix protein 1
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    corresponding to Human Extracellular matrix protein 1 aa 100-200.
    Database link: Q16610

  • Positive control
    • MDA MB 435 cell line lysate. Human skin cell lysate. A375 cell line lysate. Human breast carcinoma tissue. Human kidney tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab126629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.
    • Tissue specificity
      Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin; wide expression is observed throughout the dermis with minimal expression in the epidermis.
    • Involvement in disease
      Defects in ECM1 are the cause of lipoid proteinosis (LiP) [MIM:247100]; also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • ECM 1 antibody
      • Ecm1 antibody
      • ECM1_HUMAN antibody
      • Extracellular matrix protein 1 antibody
      • Secretory component p85 antibody
      • URBWD antibody
      see all

    Images

    • All lanes : Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629) at 1/1000 dilution

      Lane 1 : MDA MB 435 cell line lysate
      Lane 2 : Human skin cell lysate
      Lane 3 : A375 cell line lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP conjugated antibodty at 1/2000 dilution

      Predicted band size : 61 kDa
    • ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.

    • ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Ziegler YS  et al. Integration of Breast Cancer Secretomes with Clinical Data Elucidates Potential Serum Markers for Disease Detection, Diagnosis, and Prognosis. PLoS One 11:e0158296 (2016). WB . Read more (PubMed: 27355404) »

    See 1 Publication for this product

    Customer reviews and Q&As

    I have had a look at the nature of this protein: Extracellular matrix protein 1 (http://www.uniprot.org/uniprot/Q16610)

    Unfortunately, I do not think it is scientifically to detect only ECM1b without detecting ECM1a.

    The difference ...

    Read More

    Thank you for contacting us.

    In general, the selection of the immunogen varies from antibody to antibody. Some antibodies are raised against synthetic peptides (based ona protein sequence which was derived originally from a DNA sequence), so...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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