Overview

  • Product name
  • Description
    Rabbit polyclonal to EYA1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide: PYSYQMQGSS FTTSSGIYTG NNSLTNSSGF NSSQQDYPSY PSFGQGQYAQ, corresponding to internal sequence amino acids 179-228 of Human EYA1

  • Positive control
    • Fetal Brain Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab85009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Seems to coactivate SIX2, SIX4 and SIX5. May be required for normal development of branchial arches, ear and kidney.
  • Tissue specificity
    In the embryo, highly expressed in kidney with lower levels in brain. Weakly expressed in lung. In the adult, highly expressed in heart and skeletal muscle. Weakly expressed in brain and liver. No expression in eye or kidney.
  • Involvement in disease
    Defects in EYA1 are the cause of branchiootorenal syndrome type 1 (BOR1) [MIM:113650]; also known as Melnick-Fraser syndrome. BOR is an autosomal dominant disorder manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to mondini type cochlear defect and stapes fixation. Penetrance of BOR syndrome is high, although expressivity can be extremely variable.
    Defects in EYA1 are the cause of otofaciocervical syndrome (OFCS) [MIM:166780]. The syndrome is characterized by trophic alterations of the facies and shoulder girdle in addition to the malformations seen in BOR.
    Defects in EYA1 are the cause of branchiootic syndrome type 1 (BOS1) [MIM:602588]; also known as BO syndrome type 1 or branchiootic dysplasia. Individuals with BOS1 are affected by the same branchial and otic anomalies as those seen in individuals with BOR1, but lack renal anomalies.
  • Sequence similarities
    Belongs to the HAD-like hydrolase superfamily. EYA family.
  • Developmental stage
    Detected in cytoplasm of somite cells at the beginning of fourth week of development. Detected in cytoplasm of limb bud cell between the sixth and eighth week of development.
  • Post-translational
    modifications
    Sumoylated by SUMO1.
  • Cellular localization
    Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
  • Information by UniProt
  • Database links
  • Alternative names
    • BOP antibody
    • BOR antibody
    • BOS1 antibody
    • EYA transcriptional coactivator and phosphatase 1 antibody
    • Eya1 antibody
    • EYA1_HUMAN antibody
    • Eyes absent 1 antibody
    • Eyes absent 1 homolog antibody
    • Eyes absent homolog 1 (Drosophila) antibody
    • Eyes absent homolog 1 antibody
    • Eyes absent homolog1 antibody
    • MGC141875 antibody
    • OFC1 antibody
    see all

Anti-EYA1 antibody images

  • Anti-EYA1 antibody (ab85009) at 1 µg/ml + Fetal brain lysate at 10 µg

    Predicted band size : 65 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling EYA1 with ab85009 at 5.0µg/ml.

References for Anti-EYA1 antibody (ab85009)

This product has been referenced in:
  • Wu K  et al. EYA1 phosphatase function is essential to drive breast cancer cell proliferation through cyclin D1. Cancer Res 73:4488-99 (2013). Read more (PubMed: 23636126) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (differentiated induced pluripotent stem cell)
Permeabilization
Yes - 0,25% Triton X-100
Specification
differentiated induced pluripotent stem cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

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Verified customer

Submitted Jun 19 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Specification
Brain
Blocking step
Heat-Inactivated Normal Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 24 2015

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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I just checked and the Abreview you submitted for ab85009,has been published, therefore your testing discount code is ready to use. If there is anything else I can help you with, please let me know

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Eya1-3xFLAG expressed in 293T cells)
Loading amount
30 µg
Specification
Mouse Eya1-3xFLAG expressed in 293T cells
Treatment
Mouse Eya1-3xFLAG plasmid transiently transfected
Gel Running Conditions
Reduced Denaturing (10% Laemmli gel)
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Dec 13 2011

DISCOUNT CODE: ********* Expiration date: ****** I am very pleased to hear you would like to accept our offer and test ab85009 in mouse samples. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please su...

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