Overview

  • Product nameAnti-EYA1 antibody
    See all EYA1 primary antibodies
  • Description
    Goat polyclonal to EYA1
  • SpecificityExpected to recognize all isoforms.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide:

    C-TDPTAEYSTIHSP

    , corresponding to internal sequence amino acids 271-283 of Human EYA1 (NP_000494.2).

  • Positive control
    • HEK293 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa.

Target

  • FunctionTyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Seems to coactivate SIX2, SIX4 and SIX5. May be required for normal development of branchial arches, ear and kidney.
  • Tissue specificityIn the embryo, highly expressed in kidney with lower levels in brain. Weakly expressed in lung. In the adult, highly expressed in heart and skeletal muscle. Weakly expressed in brain and liver. No expression in eye or kidney.
  • Involvement in diseaseDefects in EYA1 are the cause of branchiootorenal syndrome type 1 (BOR1) [MIM:113650]; also known as Melnick-Fraser syndrome. BOR is an autosomal dominant disorder manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to mondini type cochlear defect and stapes fixation. Penetrance of BOR syndrome is high, although expressivity can be extremely variable.
    Defects in EYA1 are the cause of otofaciocervical syndrome (OFCS) [MIM:166780]. The syndrome is characterized by trophic alterations of the facies and shoulder girdle in addition to the malformations seen in BOR.
    Defects in EYA1 are the cause of branchiootic syndrome type 1 (BOS1) [MIM:602588]; also known as BO syndrome type 1 or branchiootic dysplasia. Individuals with BOS1 are affected by the same branchial and otic anomalies as those seen in individuals with BOR1, but lack renal anomalies.
  • Sequence similaritiesBelongs to the HAD-like hydrolase superfamily. EYA family.
  • Developmental stageDetected in cytoplasm of somite cells at the beginning of fourth week of development. Detected in cytoplasm of limb bud cell between the sixth and eighth week of development.
  • Post-translational
    modifications
    Sumoylated by SUMO1.
  • Cellular localizationCytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
  • Information by UniProt
  • Database links
  • Alternative names
    • BOP antibody
    • BOR antibody
    • Eya1 antibody
    • EYA1_HUMAN antibody
    • Eyes absent 1 antibody
    • Eyes absent 1 homolog antibody
    • Eyes absent homolog 1 (Drosophila) antibody
    • Eyes absent homolog 1 antibody
    • Eyes absent homolog1 antibody
    • MGC141875 antibody
    see all

Anti-EYA1 antibody images

References for Anti-EYA1 antibody (ab99186)

ab99186 has not yet been referenced specifically in any publications.

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