Overview

  • Product nameAnti-EYA2 antibody
    See all EYA2 primary antibodies
  • Description
    Rabbit polyclonal to EYA2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Rabbit, Horse, Cow, Cat, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 200-249 (EAPHNVSQSS ESLAGDYNTH NGPSTPAKEG DTDRPHRASD GKLRGRSKRS) of Rat EYA2 (NP_569111).

  • Positive control
    • Rat muscle lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab116075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionTyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1. Seems to coactivate SIX2, SIX4 and SIX5. Together with SIX1 and DACH2 seem to be involved in myogenesis. May be involved in development of the eye. Interaction with GNAZ and GNAI2 prevents nuclear translocation and transcriptional activity.
  • Tissue specificityHighest expression in muscle with lower levels in kidney, placenta, pancreas, brain and heart.
  • Sequence similaritiesBelongs to the HAD-like hydrolase superfamily. EYA family.
  • Developmental stageAt the begin of fourth week of development detected in cytoplasm of somite cells. Between the sixth and eighth week of development detected in cytoplasm of limb bud cells.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • EAB 1 antibody
    • EAB1 antibody
    • EYA 2 antibody
    • EYA2 antibody
    • EYA2_HUMAN antibody
    • eyes absent 2 homolog (Drosophila) antibody
    • Eyes absent homolog 2 (Drosophila) antibody
    • Eyes absent homolog 2 antibody
    • Translation of this uORF probably lowers the translation efficiency of EYA2 antibody
    see all

Anti-EYA2 antibody images

  • Anti-EYA2 antibody (ab116075) at 1 µg/ml + Rat muscle lysate at 10 µg

    Predicted band size : 58 kDa

References for Anti-EYA2 antibody (ab116075)

ab116075 has not yet been referenced specifically in any publications.

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