• Product name
  • Description
    Rabbit polyclonal to EYA3
  • Specificity
    ab22835 recognises EYA3
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Bird
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:


  • Positive control
    • HepG2 cell lysate, human skeletal muscle tissue sections. IF/ICC: HeLa cell line



Our Abpromise guarantee covers the use of ab22835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.05 - 1 µg/ml. Predicted molecular weight: 62 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.


  • Function
    Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity (By similarity). May be involved in development of the eye.
  • Sequence similarities
    Belongs to the HAD-like hydrolase superfamily. EYA family.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR. Ser-266 phosphorylation is required for localization at sites of DNA damage and directing interaction with H2AX.
  • Cellular localization
    Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks (DSBs). With decreasing efficiency, translocalized to the nucleus by SIX2 and SIX5, and SIX4, respectively.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI844637 antibody
    • DKFZp686C132 antibody
    • EYA3 antibody
    • EYA3_HUMAN antibody
    • Eyes absent 3 antibody
    • Eyes absent 3 homolog (Drosophila) antibody
    • Eyes absent homolog 3 antibody
    • OTTMUSP00000010370 antibody
    • RP11-460I13.4 antibody
    • RP23-442A20.1 antibody
    see all

Anti-EYA3 antibody images

  • ICC/IF image of ab22835 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22835, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-EYA3 antibody (ab22835) at 0.1 µg/ml + HepG2 cell lysate with 5% skim milk in PBS

    HRP conjugated anti-rabbit IgG at 1/50000 dilution

    Predicted band size : 62 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
    12% gel concentration.
  • Immunohistochemistry detection of EYA3 in paraffin embedded human skeletal muscle tissue section. ab22835 was used at 4 ug/ml. Arrows indicate positively labelled muscle cells.

References for Anti-EYA3 antibody (ab22835)

This product has been referenced in:
  • Majumdar G  et al. A photoperiodic molecular response in migratory redheaded bunting exposed to a single long day. Gen Comp Endocrinol 204C:104-113 (2014). IHC-P ; Bird . Read more (PubMed: 24837606) »

See 1 Publication for this product

Product Wall

Thank you for your patience. My colleague has been able to add the following peptides to our catalog:

Ab77481 corresponding peptide Ab131396

Ab102926 corresponding peptide Ab131397

Ab22835 corresponding peptide AB131398 ...

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Thank you for your reply.

I will be working on adding these peptides to our catalog, and it may take a couple of days.

Regardingthe TSH beta antibody ab80784, the immunogen was purified human TSH beta. We don't have the beta subuni...

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Thank you for bringing this matter to my attention. I have received the correct immunogenic peptide sequence from the originator of ab22835 and it is: "QSRKNMTSKNRGKRKADATSSQDSELERVFLWDLDETIIIFHSLLTGSYA" I apologize for the mistake in the data...

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