Overview

  • Product nameAnti-EYA3 antibody
    See all EYA3 primary antibodies
  • Description
    Rabbit polyclonal to EYA3
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Sheep, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human EYA3 aa 1-30.
    Database link: Q99504

  • Positive control
    • HeLa, 293T cell lysate, IHC controls:breast, colon and prostate carcinoma

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab95876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 63 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/500.

Target

  • FunctionTyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity (By similarity). May be involved in development of the eye.
  • Sequence similaritiesBelongs to the HAD-like hydrolase superfamily. EYA family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR. Ser-266 phosphorylation is required for localization at sites of DNA damage and directing interaction with H2AX.
  • Cellular localizationCytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks (DSBs). With decreasing efficiency, translocalized to the nucleus by SIX2 and SIX5, and SIX4, respectively.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI844637 antibody
    • DKFZp686C132 antibody
    • EYA3 antibody
    • EYA3_HUMAN antibody
    • Eyes absent 3 antibody
    • Eyes absent 3 homolog (Drosophila) antibody
    • Eyes absent homolog 3 antibody
    • OTTMUSP00000010370 antibody
    • RP11-460I13.4 antibody
    • RP23-442A20.1 antibody
    see all

Anti-EYA3 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling EYA3 with ab95876 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-EYA3 antibody (ab95876) at 0.04 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 63 kDa


    Exposure time : 30 seconds
  • Detection of human Eya3 by Western Blot of Immunoprecipitate. ab95876 at 0.04µg/ml staining Eya3 in HeLa cell lysate immunoprecipitated using ab95876 at 3µg/mg (1mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

References for Anti-EYA3 antibody (ab95876)

ab95876 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Human Cell (Human Retinal Microvascular Enothelial)
Specification Human Retinal Microvascular Enothelial
Permeabilization Yes - 0.2% Triton X-100
Fixative Paraformaldehyde
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Submitted Jul 09 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"