Overview

  • Product nameAnti-EYA3 antibody
    See all EYA3 primary antibodies
  • Description
    Rabbit polyclonal to EYA3
  • Tested applicationsSuitable for: ICC/IF, IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human EYA3 aa 30-60.
    Database link: Q99504

  • Positive control
    • HeLa whole cell lysate. IHC controls: Breast Carcinoma, Colon Carcinoma

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab95877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/2000.
IHC-P 1/500 - 1/2000.
IP Use at 2-5 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionTyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity (By similarity). May be involved in development of the eye.
    • Sequence similaritiesBelongs to the HAD-like hydrolase superfamily. EYA family.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR. Ser-266 phosphorylation is required for localization at sites of DNA damage and directing interaction with H2AX.
    • Cellular localizationCytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks (DSBs). With decreasing efficiency, translocalized to the nucleus by SIX2 and SIX5, and SIX4, respectively.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI844637 antibody
      • DKFZp686C132 antibody
      • EYA3 antibody
      • EYA3_HUMAN antibody
      • Eyes absent 3 antibody
      • Eyes absent 3 homolog (Drosophila) antibody
      • Eyes absent homolog 3 antibody
      • OTTMUSP00000010370 antibody
      • RP11-460I13.4 antibody
      • RP23-442A20.1 antibody
      see all

    References for Anti-EYA3 antibody (ab95877)

    ab95877 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"