Overview

  • Product nameAnti-EYA4 antibody
    See all EYA4 primary antibodies
  • Description
    Rabbit polyclonal to EYA4
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids of Human EYA4

  • Positive control
    • A2058 cell lysate. Human skeletal muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab102648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 70 kDa. It only recognizes Isoform 3 with predicted MW 48.7 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionTyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. May be involved in development of the eye.
  • Tissue specificityHighly expressed in heart and skeletal muscle.
  • Involvement in diseaseDefects in EYA4 are the cause of deafness autosomal dominant type 10 (DFNA10) [MIM:601316]. DFNA10 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
    Defects in EYA4 are the cause of cardiomyopathy dilated type 1J (CMD1J) [MIM:605362]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similaritiesBelongs to the HAD-like hydrolase superfamily. EYA family.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CMD1J antibody
    • Deafness, autosomal dominant 10 antibody
    • DFNA 10 antibody
    • DFNA10 antibody
    • dJ78N10.1 (eyes absent (Drosophila) homolog 4) antibody
    • dJ78N10.1 (eyes absent) antibody
    • EYA 4 antibody
    • eya4 antibody
    • EYA4_HUMAN antibody
    • Eyes absent 4 antibody
    • Eyes absent homolog 4 (Drosophila) antibody
    • Eyes absent homolog 4 antibody
    • HGNC:3522 antibody
    • OTTHUMP00000040267 antibody
    see all

Anti-EYA4 antibody images

  • Anti-EYA4 antibody (ab102648) at 1/100 dilution + A2058 cell lysate at 35 µg

    Predicted band size : 70 kDa
  • ab102648 staining formalin fixed human skeletal muscle at 1/50 dilution.

References for Anti-EYA4 antibody (ab102648)

ab102648 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry and your interest. ab102648 has a potential to recognize all four isoforms of EYA4 if all isoforms are present at the same time and at detectable levels. However, it only recognizes isoform 3 with predicted MW 48.7 kDa. ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"