This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

  • Product name
    Anti-EYA4 antibody - Aminoterminal end
    See all EYA4 primary antibodies
  • Description
    Goat polyclonal to EYA4 - Aminoterminal end

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Specificity
    This antibody is expected to recognise isoforms a, c and d (NP_004091.2, NP_742102.1 and NP_742103.1 respectively).
  • Species reactivity

    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    EDSQDLNEQSVKKTC

    , corresponding to N terminal amino acids 2-16 of Human EYA4

Properties

Applications

  • Application notes
    This antibody gave a positive result in ELISA against the immunizing peptide. Antibody detection limit dilution 1:128,000.

    Western Blot: Preliminary experiments gave an approx 50kDa band in Human and Mouse Brain lysates and in Pituitary Mouse cell line NIH 3T3 after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated sizes of 69.5kDa, 67.1kDa and 69.4kDa according to NP_004091.2, NP_742102.1 and NP_742103.1 respectively. The 50kDa band was successfully blocked by incubation with the immunizing peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. May be involved in development of the eye.
    • Tissue specificity
      Highly expressed in heart and skeletal muscle.
    • Involvement in disease
      Defects in EYA4 are the cause of deafness autosomal dominant type 10 (DFNA10) [MIM:601316]. DFNA10 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
      Defects in EYA4 are the cause of cardiomyopathy dilated type 1J (CMD1J) [MIM:605362]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • Sequence similarities
      Belongs to the HAD-like hydrolase superfamily. EYA family.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CMD1J antibody
      • Deafness, autosomal dominant 10 antibody
      • DFNA 10 antibody
      • DFNA10 antibody
      • dJ78N10.1 (eyes absent (Drosophila) homolog 4) antibody
      • dJ78N10.1 (eyes absent) antibody
      • EYA 4 antibody
      • eya4 antibody
      • EYA4_HUMAN antibody
      • Eyes absent 4 antibody
      • Eyes absent homolog 4 (Drosophila) antibody
      • Eyes absent homolog 4 antibody
      • HGNC:3522 antibody
      • OTTHUMP00000040267 antibody
      see all

    References

    This product has been referenced in:
    • Huang S  et al. Epigenetic Silencing of Eyes Absent 4 Gene by Acute Myeloid Leukemia 1-Eight-twenty-one Oncoprotein Contributes to Leukemogenesis in t(8;21) Acute Myeloid Leukemia. Chin Med J (Engl) 129:1355-62 (2016). WB . Read more (PubMed: 27231175) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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