The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).Can be blocked with Human EZH1 peptide (ab22954).
FunctionPolycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. EZ subfamily. Contains 1 SET domain.
Cellular localizationNucleus. Colocalizes with trimethylated 'Lys-27' of histone H3.