Anti-EZH1 antibody - N-terminal (ab137693)
Key features and details
- Rabbit polyclonal to EZH1 - N-terminal
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EZH1 antibody - N-terminal
See all EZH1 primary antibodies -
Description
Rabbit polyclonal to EZH1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 1-44 of Human EZH1 (Uniprot Q92800)
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, Molt-4 and Raji cells; HeLa cells; H1299 xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 85 kDa.
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or Tris-EDTA buffer pH8.0
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 85 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or Tris-EDTA buffer pH8.0 |
Target
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Function
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. -
Sequence similarities
Belongs to the histone-lysine methyltransferase family. EZ subfamily.
Contains 1 SET domain. -
Cellular localization
Nucleus. Colocalizes with trimethylated 'Lys-27' of histone H3. - Information by UniProt
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Database links
- Entrez Gene: 2145 Human
- Omim: 601674 Human
- SwissProt: Q92800 Human
- Unigene: 194669 Human
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Alternative names
- Enhancer of zeste homolog 1 (Drosophila) antibody
- Enhancer of zeste homolog 1 antibody
- ENX-2 antibody
see all
Images
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Anti-EZH1 antibody - N-terminal (ab137693) at 1/1000 dilution + whole cell lysate prepared from HepG2 cells at 30 µg
Predicted band size: 85 kDa7.5% SDS PAGE.
Secondary antibody - anti-rabbit HRP (ab6721)
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab137693 at 1/1000 dilution.
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Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using ab137693 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab137693 has been referenced in 2 publications.
- Schümann FL et al. Divergent Effects of EZH1 and EZH2 Protein Expression on the Prognosis of Patients with T-Cell Lymphomas. Biomedicines 9:N/A (2021). PubMed: 34944658
- Zhang W et al. miR-17-5p down-regulation contributes to erlotinib resistance in non-small cell lung cancer cells. J Drug Target 25:125-131 (2017). PubMed: 27633093