Anti-EZH1 antibody - N-terminal (ab137693)

Overview

  • Product name
    Anti-EZH1 antibody - N-terminal
    See all EZH1 primary antibodies
  • Description
    Rabbit polyclonal to EZH1 - N-terminal
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-44 of Human EZH1 (Uniprot Q92800)

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, Molt-4 and Raji cells; HeLa cells; H1299 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab137693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 85 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or Tris-EDTA buffer pH8.0

Target

  • Function
    Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
  • Sequence similarities
    Belongs to the histone-lysine methyltransferase family. EZ subfamily.
    Contains 1 SET domain.
  • Cellular localization
    Nucleus. Colocalizes with trimethylated 'Lys-27' of histone H3.
  • Information by UniProt
  • Database links
  • Alternative names
    • Enhancer of zeste homolog 1 (Drosophila) antibody
    • Enhancer of zeste homolog 1 antibody
    • ENX-2 antibody
    • ENX2 antibody
    • Ezh1 antibody
    • EZH1_HUMAN antibody
    • Histone-lysine N-methyltransferase EZH1 antibody
    see all

Images

  • Anti-EZH1 antibody - N-terminal (ab137693) at 1/1000 dilution + whole cell lysate prepared from HepG2 cells at 30 µg

    Predicted band size : 85 kDa

    7.5% SDS PAGE.

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab137693 at 1/1000 dilution.
  • Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using ab137693 at 1/500 dilution.

References

ab137693 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (Mouse stem cells)
Specification
Mouse stem cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Oct 14 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Mouse Cell lysate - whole cell (mouse stem cells)
Specification
mouse stem cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Sep 20 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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