Overview

  • Product name
    Anti-EZH1 antibody - N-terminal
    See all EZH1 primary antibodies
  • Description
    Rabbit polyclonal to EZH1 - N-terminal
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human EZH1 (N terminal). The exact sequence is proprietary. AAC50778
    Database link: Q92800

  • Positive control
    • Mouse lung tissue lysate and Human lung tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab176115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 85 kDa.
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
  • Sequence similarities
    Belongs to the histone-lysine methyltransferase family. EZ subfamily.
    Contains 1 SET domain.
  • Cellular localization
    Nucleus. Colocalizes with trimethylated 'Lys-27' of histone H3.
  • Information by UniProt
  • Database links
  • Alternative names
    • Enhancer of zeste homolog 1 (Drosophila) antibody
    • Enhancer of zeste homolog 1 antibody
    • ENX-2 antibody
    • ENX2 antibody
    • Ezh1 antibody
    • EZH1_HUMAN antibody
    • Histone-lysine N-methyltransferase EZH1 antibody
    see all

Images

  • Immunohistochemical analysis of Human lung tissue labeling EZH1 with ab176115  at 5 μg/ml dilution.

  • Lane 1 : Anti-EZH1 antibody - N-terminal (ab176115) at 1 µg/ml
    Lane 2 : Anti-EZH1 antibody - N-terminal (ab176115) at 2 µg/ml

    Lane 1 : mouse lung tissue lysate
    Lane 2 : mouse lung tissue lysate

    Lysates/proteins at 15 µg per lane.

    Developed using the ECL technique

    Predicted band size : 85 kDa

References

This product has been referenced in:
  • Li J  et al. EZH2-mediated H3K27 trimethylation mediates neurodegeneration in ataxia-telangiectasia. Nat Neurosci 16:1745-53 (2013). Read more (PubMed: 24162653) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12% Bis-Tris Gel, MES running buffer)
Sample
Mouse Tissue lysate - whole (Whole brain tissue lysate)
Specification
Whole brain tissue lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Sep 12 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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