Overview

  • Product nameAnti-Ezrin antibody [3C12]
    See all Ezrin primary antibodies
  • Description
    Mouse monoclonal [3C12] to Ezrin
  • Tested applicationsSuitable for: IHC-FoFr, WB, ICC/IF, IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Cow, Human, Pig, Kangaroo, Monkey
  • Immunogen

    Recombinant fragment, corresponding to amino acids 362-585 of Human Ezrin.

  • Positive control
    • Lung.

Properties

Applications

Our Abpromise guarantee covers the use of ab4069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 22427845
WB 1/100 - 1/750. Predicted molecular weight: 69 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 17311105
IHC-P 1/50 - 1/100.
IHC-Fr 1/50 - 1/100.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionProbably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
  • Tissue specificityExpressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
  • Sequence similaritiesContains 1 FERM domain.
  • Developmental stageVery strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
  • Post-translational
    modifications
    Phosphorylated by tyrosine-protein kinases.
  • Cellular localizationApical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
  • Information by UniProt
  • Database links
  • Alternative names
    • Villin 2 ezrin antibody
    • CVIL antibody
    • CVL antibody
    • Cytovillin 2 antibody
    • Cytovillin antibody
    • DKFZp762H157 antibody
    • Epididymis secretory protein Li 105 antibody
    • EZR antibody
    • EZRI_HUMAN antibody
    • Ezrin antibody
    • FLJ26216 antibody
    • HEL S 105 antibody
    • MGC1584 antibody
    • p81 antibody
    • VIL 2 antibody
    • VIL2 antibody
    • Villin 2 (ezrin) antibody
    • Villin 2 antibody
    • Villin-2 antibody
    • Villin2 antibody
    see all

Anti-Ezrin antibody [3C12] images

  • Anti-Ezrin antibody [3C12] (ab4069) at 1/500 dilution + Mouse bEnd.3 whole cell lysate at 20 µg

    Secondary
    HRP-conjugated goat anti-mouse IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 69 kDa
    Observed band size : 81 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Rat lung epithelial microvilli stained with ab4069 (anti-Ezrin) in frozen sections. Review by Marko Nykanen submitted 1 July 2004.
  • ab4069 at 1/100 dilution staining human HeLa cells by ICC/IF. The cells were formaldehyde fixed and blocked with BSA/goat serum prior to incubation with the antibody for 30 minutes. An Alexa-Fluor ® 568 conjugated goat anti-mouse IgG was used as the secondary.

    See Abreview

  • ab4069 staining human lung by IHC-P.
  • Overlay histogram showing SH-SY5Y cells stained with ab4069 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab4069, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung adenocarcinoma tissue, staining Ezrin with ab4069.
  • Immunohistochemical analysis of murine retina tissue, staining Ezrin (red) with ab4069.

    Tissue was blocked with blocking solution before incubation with primary antibody (1/50) overnight at 4°C. An AlexaFluor®555-conjugated donkey anti-mouse IgG (1/20) was used as the secondary antibody.

References for Anti-Ezrin antibody [3C12] (ab4069)

This product has been referenced in:

See all 11 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Pig Cell (PAEC)
Specification PAEC
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Paraformaldehyde
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Submitted Sep 11 2014

Application Western blot
Loading amount 40 µg
Gel Running Conditions Non-reduced Denaturing (SDS gel 10%)
Sample Human Cell lysate - whole cell (Ovarian cancer)
Specification Ovarian cancer
Treatment 50uM Metformin for 24hr
Blocking step Milk as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 01 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (HUVEC)
Specification HUVEC
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Jul 17 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (bEnd.3)
Specification bEnd.3
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Jul 16 2014

Thank you for your enquiry. I am pleased to help answer your questions:

1. Any IHC-P testing images we have will be available on the individual online datasheets. Please let me know if there is not one available for the antibody you are inte...

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Thank you for your enquiry and happy new year to you. I am sorry to confirm that because we carry over 70,000 products, it is not feasible for us to keep small sample sizes of our products to send to customers. We are happy to reassure our customers th...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Blocking step BSA with goat serum as blocking agent for 30 minute(s) · Concentration: 10%
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Submitted Dec 07 2006

Thank you for your enquiry. The concentration is 100 ug/ml. If you have any additional questions, please contact us again.

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you ...

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The concentration is 100 ug/ml. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"