Overview

  • Product nameAnti-Ezrin antibody
    See all Ezrin primary antibodies
  • Description
    Rabbit polyclonal to Ezrin
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Chicken
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human Ezrin.

    (Peptide available as ab42317.)

  • Positive control
    • ab41672 gave a positive result in the following whole cell lysates: Hela, Jurkat, A431, HEK293, HepG2, MCF7, U20S.

Properties

Applications

Our Abpromise guarantee covers the use of ab41672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionProbably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
  • Tissue specificityExpressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
  • Sequence similaritiesContains 1 FERM domain.
  • Developmental stageVery strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
  • Post-translational
    modifications
    Phosphorylated by tyrosine-protein kinases.
  • Cellular localizationApical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
  • Information by UniProt
  • Database links
  • Alternative names
    • Villin 2 ezrin antibody
    • CVIL antibody
    • CVL antibody
    • Cytovillin 2 antibody
    • Cytovillin antibody
    • DKFZp762H157 antibody
    • Epididymis secretory protein Li 105 antibody
    • EZR antibody
    • EZRI_HUMAN antibody
    • Ezrin antibody
    • FLJ26216 antibody
    • HEL S 105 antibody
    • MGC1584 antibody
    • p81 antibody
    • VIL 2 antibody
    • VIL2 antibody
    • Villin 2 (ezrin) antibody
    • Villin 2 antibody
    • Villin-2 antibody
    • Villin2 antibody
    see all

Anti-Ezrin antibody images

  • ab41672 staining Ezrin in undifferentiated human embryonic stem cells on a murine embryonic fibroblast (MEFs) feeding layer by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized and blocked with 1% serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/250 in 1% serum, 0.1% triton, 0.1% BSA + PBS) for 16 hours at 4°C. An Alexa Flour® 488-conjugated donkey anti-rabbit IgG polyclonal was used as the secondary antibody at a dilution of 1/500.

  • All lanes : Anti-Ezrin antibody (ab41672) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 69 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    The observed molecular weight of 80 kDa is in agreement with previous findings (Zhu et al, Am J Physiol Cell Physiol. 2005 Jun;288(6):C1242-54.; PMID: 15788482).

  • Ezrin was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal Ezrin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab41672.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to anti-Rabbit HRP (IgG light chain) (ab99697).
    Band: 80kDa: Ezrin

  • IHC image of Ezrin staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41672, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-Ezrin antibody (ab41672)

This product has been referenced in:
  • Perisic L  et al. Schip1 is a novel podocyte foot process protein that mediates actin cytoskeleton rearrangements and forms a complex with Nherf2 and ezrin. PLoS One 10:e0122067 (2015). Read more (PubMed: 25807495) »
  • Lázaro-Diéguez F  et al. Par1b links lumen polarity with LGN-NuMA positioning for distinct epithelial cell division phenotypes. J Cell Biol 203:251-64 (2013). Read more (PubMed: 24165937) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (undifferentiated Human ES cells on MEFs)
Permeabilization Yes - see blocking buffer
Specification undifferentiated Human ES cells on MEFs
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Fixative Paraformaldehyde
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Submitted Feb 22 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HUES7 ES cells)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 20 µg
Specification HUES7 ES cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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Submitted Feb 04 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEPG2)
Specification HEPG2
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
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Submitted Nov 18 2015

Application Western blot
Sample Human Cell lysate - whole cell (HEPG2)
Gel Running Conditions Reduced Denaturing (12.5)
Loading amount 20 µg
Specification HEPG2
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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Submitted Nov 13 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Sample Mouse Cell (mouse embryonic stem cell)
Specification mouse embryonic stem cell
Permeabilization Yes - 0.1% triton x
Fixative methanol:acetone 1:1
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Submitted Dec 20 2013

Application Western blot
Loading amount 1e+006 cells
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Specification mouse embryonic stem cell
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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Submitted Dec 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Adrenal (human, mouse and rat))
Specification Adrenal (human, mouse and rat)
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization No
Blocking step Sequential peroxidase and protein block (prediluted) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
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Verified customer

Submitted Apr 30 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"