• Product nameAnti-Ezrin antibody
    See all Ezrin primary antibodies
  • Description
    Rabbit polyclonal to Ezrin
  • Specificityab53065 detects endogenous levels of total Ezrin protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide derived from human Ezrin.

  • Positive control
    • Human brain tissue and NIH/3T3 cell extracts.



Our Abpromise guarantee covers the use of ab53065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.


  • FunctionProbably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
  • Tissue specificityExpressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
  • Sequence similaritiesContains 1 FERM domain.
  • Developmental stageVery strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
  • Post-translational
    Phosphorylated by tyrosine-protein kinases.
  • Cellular localizationApical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
  • Information by UniProt
  • Database links
  • Alternative names
    • Villin 2 ezrin antibody
    • CVIL antibody
    • CVL antibody
    • Cytovillin 2 antibody
    • Cytovillin antibody
    • DKFZp762H157 antibody
    • Epididymis secretory protein Li 105 antibody
    • EZR antibody
    • EZRI_HUMAN antibody
    • Ezrin antibody
    • FLJ26216 antibody
    • HEL S 105 antibody
    • MGC1584 antibody
    • p81 antibody
    • VIL 2 antibody
    • VIL2 antibody
    • Villin 2 (ezrin) antibody
    • Villin 2 antibody
    • Villin-2 antibody
    • Villin2 antibody
    see all

Anti-Ezrin antibody images

  • All lanes : Anti-Ezrin antibody (ab53065) at 1/500 dilution

    Lane 1 : NIH/3T3 cell extract
    Lane 2 : NIH/3T3 cell extract with immunising peptide

    Predicted band size : 69 kDa
    Observed band size : 69 kDa
  • ab53065 at 1/50 dilution staining Ezrin in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

References for Anti-Ezrin antibody (ab53065)

ab53065 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (western blot)
Loading amount 40 µg
Specification western blot
Gel Running Conditions Reduced Denaturing (4-12% gradient gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Sep 29 2011