Overview

  • Product name
    Anti-Ezrin antibody [EP924Y]
    See all Ezrin primary antibodies
  • Description
    Rabbit monoclonal [EP924Y] to Ezrin
  • Specificity
    ab75840 detects both phosphorylated and non phosphorylated Ezrin.
  • Tested applications
    Suitable for: WB, ICC, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues surrounding tyrosine 145 of human Ezrin

  • Positive control
    • 293, A431 and SH-SY5Y cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab75840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
ICC 1/100 - 1/250.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
    • Tissue specificity
      Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
    • Sequence similarities
      Contains 1 FERM domain.
    • Developmental stage
      Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
    • Post-translational
      modifications
      Phosphorylated by tyrosine-protein kinases.
    • Cellular localization
      Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
    • Information by UniProt
    • Database links
    • Alternative names
      • Villin 2 ezrin antibody
      • CVIL antibody
      • CVL antibody
      • Cytovillin 2 antibody
      • Cytovillin antibody
      • DKFZp762H157 antibody
      • Epididymis secretory protein Li 105 antibody
      • EZR antibody
      • EZRI_HUMAN antibody
      • Ezrin antibody
      • FLJ26216 antibody
      • HEL S 105 antibody
      • MGC1584 antibody
      • p81 antibody
      • VIL 2 antibody
      • VIL2 antibody
      • Villin 2 (ezrin) antibody
      • Villin 2 antibody
      • Villin-2 antibody
      • Villin2 antibody
      see all

    Images

    • All lanes : Anti-Ezrin antibody [EP924Y] (ab75840) at 1/10000 dilution

      Lane 1 : 293 cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 69 kDa
      Observed band size : 81 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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