Ezrin overexpression 293T lysate (whole cell) (ab94091)

Overview

  • Product nameEzrin overexpression 293T lysate (whole cell)
  • Description
    Ezrin overexpression lysate
  • General notesab94091 is a 293T cell transfected lysate in which Human Ezrin has been transiently over-expressed using a pCMV-Ezrin. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. Tissue specificity: Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied. Similarity: Contains 1 FERM domain. Developmental stage: Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain. PTM: Phosphorylated by tyrosine-protein kinases.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Ezrin overexpression 293T lysate (whole cell) images

  • ab94091 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Ezrin antibody (ab76872) at 1/500 dilution

    Lane 1 : Ezrin overexpression 293T lysate (whole cell) (ab94091)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Ezrin overexpression 293T lysate (whole cell) (ab94091)

ab94091 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"