Anti-Ezrin (phospho Y146) antibody (ab92557)


  • Product nameAnti-Ezrin (phospho Y146) antibody
    See all Ezrin primary antibodies
  • Description
    Rabbit polyclonal to Ezrin (phospho Y146)
  • SpecificityDetects endogenous levels of Ezrin only when phosphorylated at Tyrosine 146.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from Human Ezrin around the phosphorylation site of Tyrosine 146 (SGYPLS).

  • Positive control
    • Extracts from UV-treated 293 cells



Our Abpromise guarantee covers the use of ab92557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: 1/40000.
    WB: 1/500 - 1/1000. Predicted molecular weight: 69 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionProbably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
    • Tissue specificityExpressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
    • Sequence similaritiesContains 1 FERM domain.
    • Developmental stageVery strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
    • Post-translational
      Phosphorylated by tyrosine-protein kinases.
    • Cellular localizationApical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
    • Information by UniProt
    • Database links
    • Alternative names
      • Villin 2 ezrin antibody
      • CVIL antibody
      • CVL antibody
      • Cytovillin 2 antibody
      • Cytovillin antibody
      • DKFZp762H157 antibody
      • Epididymis secretory protein Li 105 antibody
      • EZR antibody
      • EZRI_HUMAN antibody
      • Ezrin antibody
      • FLJ26216 antibody
      • HEL S 105 antibody
      • MGC1584 antibody
      • p81 antibody
      • VIL 2 antibody
      • VIL2 antibody
      • Villin 2 (ezrin) antibody
      • Villin 2 antibody
      • Villin-2 antibody
      • Villin2 antibody
      see all

    Anti-Ezrin (phospho Y146) antibody images

    • All lanes : Anti-Ezrin (phospho Y146) antibody (ab92557) at 1/500 dilution

      Lane 1 : 293 cells (treated with UV for 15 mins)
      Lane 2 : 293 cells (treated with UV for 15 mins) with immunizing peptide at 5 µg

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 69 kDa

    References for Anti-Ezrin (phospho Y146) antibody (ab92557)

    ab92557 has not yet been referenced specifically in any publications.

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