Anti-Ezrin (phospho Y353) antibody [EP925Y] (ab52916)


  • Product nameAnti-Ezrin (phospho Y353) antibody [EP925Y]
    See all Ezrin primary antibodies
  • Description
    Rabbit monoclonal [EP925Y] to Ezrin (phospho Y353)
  • SpecificityDetects Ezrin phosphorylated on Tyrosine 353.
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phospho-specific peptide corresponding to residues surrounding Tyr353P of human Ezrin.

  • Positive control
    • Lysate of 293 cells treated with pervanadate.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab52916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200000. Predicted molecular weight: 69 kDa.
  • Application notesIs unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • FunctionProbably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
    • Tissue specificityExpressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
    • Sequence similaritiesContains 1 FERM domain.
    • Developmental stageVery strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
    • Post-translational
      Phosphorylated by tyrosine-protein kinases.
    • Cellular localizationApical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
    • Information by UniProt
    • Database links
    • Alternative names
      • Villin 2 ezrin antibody
      • CVIL antibody
      • CVL antibody
      • Cytovillin 2 antibody
      • Cytovillin antibody
      • DKFZp762H157 antibody
      • Epididymis secretory protein Li 105 antibody
      • EZR antibody
      • EZRI_HUMAN antibody
      • Ezrin antibody
      • FLJ26216 antibody
      • HEL S 105 antibody
      • MGC1584 antibody
      • p81 antibody
      • VIL 2 antibody
      • VIL2 antibody
      • Villin 2 (ezrin) antibody
      • Villin 2 antibody
      • Villin-2 antibody
      • Villin2 antibody
      see all

    Anti-Ezrin (phospho Y353) antibody [EP925Y] images

    • All lanes : Anti-Ezrin (phospho Y353) antibody [EP925Y] (ab52916) at 1/200000 dilution

      Lane 1 : 293 cell lysate untreated
      Lane 2 : 293 cell lysate treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 69 kDa
      Observed band size : 69 kDa

    References for Anti-Ezrin (phospho Y353) antibody [EP925Y] (ab52916)

    ab52916 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab52916.
    Please use the links above to contact us or submit feedback about this product.