Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

Overview

  • Product nameAnti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y]
  • Description
    Rabbit monoclonal [EP2122Y] to Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558)
  • Specificityab76247 will detect Ezrin, Radixin and Moesin. This antibody detects the phosphorylated target and not the non-phosphorylated epitope.
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IF, Dot blotmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558). A synthetic peptide corresponding to residues surrounding Thr567 (Ezrin), Thr564 (Radixin) and Thr558 (Moesin) of human Ezrin.

  • Positive control
    • IHC: human breast adenocarcinoma IF: HeLa cells Dot Blot: Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) phospho peptide and Non-phospho peptide.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    This antibody detects the phosphorylated target and not the non-phosphorylated epitope.

Properties

Applications

Our Abpromise guarantee covers the use of ab76247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 75-81 kDa (predicted molecular weight: 69 kDa).
IP 1/40.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
Dot blot 1/1000.
  • Application notesIs unsuitable for Flow Cyt.
  • Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] images

    • Immunofluorescent staining of HeLa cells using ab76247, at 1/250 dilution
    • Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma with ab76247 at 1/100 dilution.

    • Dot blot analysis of Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) phospho peptide (Lane 1), Non-phospho peptide (Lane 2), labelling Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) with ab76247 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

      Blocking and diluting buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      The sequence of non-phospho peptide: CGRDKYKTLRQIR.
      The sequence of phospho peptide: CGRDKYK-pT-LRQIR.

    References for Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

    This product has been referenced in:
    • Lööv C  et al. Identification of injury specific proteins in a cell culture model of traumatic brain injury. PLoS One 8:e55983 (2013). WB, ICC/IF ; Mouse . Read more (PubMed: 23409102) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"