Anti-F box protein 38 antibody (ab87729)

Overview

  • Product nameAnti-F box protein 38 antibody
  • Description
    Rabbit polyclonal to F box protein 38
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 825 and 875 of Human F box protein 38 (NP_995308.1).

  • Positive control
    • HeLa and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab87729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 134 kDa.
IP Use at 5-10 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-F box protein 38 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (right) and human testicular seminoma (right) tissues labelling F box protein 38 with ab87729 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-F box protein 38 antibody (ab87729) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from NIH3T3 cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 134 kDa
    Observed band size : 134 kDa
    Additional bands at : 107 kDa (possible isoform).

    Exposure time : 3 minutes
  • Detection of F box protein 38 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab87729 at 10 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References for Anti-F box protein 38 antibody (ab87729)

ab87729 has not yet been referenced specifically in any publications.

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