Anti-F4/80 antibody (ab96641)


  • Product name
  • Description
    Rabbit polyclonal to F4/80
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human F4/80 aa 626-859.
    Database link: Q14246

  • Positive control
    • A431, H1299 whole cell lysates



Our Abpromise guarantee covers the use of ab96641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 98 kDa.


  • Function
    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • Tissue specificity
    Expression is restricted to eosinophils.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all


  • All lanes : Anti-F4/80 antibody (ab96641) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 98 kDa


ab96641 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing
Mouse Cell lysate - whole cell (peritoneal macrophage)
peritoneal macrophage
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 12 2014

**Please note that you need to purchase this vial of ab74383 at list price as you normally would. After you submit your Abreview describing your experience staining rat tissue with ab74383, you can then use the discount code above to get your free...

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Thanks for your email.
ab96641 may work in histo immuno staining but this application has not yet been tested.
I hope this helps. Please contact me if you have any further questions.
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Thank you for your enquiry.
Yes, ab74383 and ab15694 both stain F4/80. ab74383 has been found to react with rat F4/80 using western blotting.
I hope this helps. Please contact me again if you have any further questions.

Thank you for your enquiry.
We have not done side by side comparisons using these two antibodies.
Please contact me if you have any further questions.

Sure, ab74383 is also a good option for testing in the promotion.
Would you like me to arrange the testing discount promotion code for testing ab74383 with your rat sections?

Thank you for your enquiry.
At this time we don't have any antibodies that have been tested for reactivity to rat F4/80 AND have been used in IHC.
You might consider ab15694, a biotinilated rat antibody that has been used successfully in IHC....

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