Anti-F4/80 antibody [BM8] (Biotin) (ab15694)


  • Product nameAnti-F4/80 antibody [BM8] (Biotin)
    See all F4/80 primary antibodies
  • Description
    Rat monoclonal [BM8] to F4/80 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: IHC-Fr, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Unfortunately, this information is considered to be commercially sensitive

  • EpitopeF4/80 antigen.
  • Positive control
    • Macrophages in spleen, lymph nodes, thymus, liver, skin. Langerhans cells in the skin. Kupffer cells in the liver.
  • General notesThe antigen is a 125kD cell surface membrane bound protein (non-reducing conditions). It is sensitive to 2 mercaptoethanol.



Our Abpromise guarantee covers the use of ab15694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 25185444
Flow Cyt Use at an assay dependent concentration. ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.


  • FunctionOrphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • Tissue specificityExpression is restricted to eosinophils.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

Anti-F4/80 antibody [BM8] (Biotin) images

  • ab15694 staining pan Macrophage in murine liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 2% milk for 10 minutes at 20°C followed by incubation with the primary antibody at a 1/4 dilution for 2 hours at 4°C.

    See Abreview

References for Anti-F4/80 antibody [BM8] (Biotin) (ab15694)

This product has been referenced in:
  • Gerlini A  et al. The role of host and microbial factors in the pathogenesis of pneumococcal bacteraemia arising from a single bacterial cell bottleneck. PLoS Pathog 10:e1004026 (2014). Flow Cyt ; Mouse . Read more (PubMed: 24651834) »
  • Gumucio J  et al. T lymphocytes are not required for the development of fatty degeneration after rotator cuff tear. Bone Joint Res 3:262-72 (2014). IHC-Fr ; Rat . Read more (PubMed: 25185444) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6.0
Permeabilization No
Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 20°C

Mr. Brendan Toohey

Verified customer

Submitted Nov 05 2010

Thank you for your enquiry and patience. I regret to inform you that this antibody has not yet been tested for application in IHC-P (paraffin sections) and so I cannot provide you with the protocol that was used. I have removed this application from th...

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