Anti-F4/80 antibody [BM8] (Biotin) (ab15694)

Overview

  • Product name
    Anti-F4/80 antibody [BM8] (Biotin)
    See all F4/80 primary antibodies
  • Description
    Rat monoclonal [BM8] to F4/80 (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: IHC-Fr, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Unfortunately, this information is considered to be commercially sensitive

  • Epitope
    F4/80 antigen.
  • Positive control
    • Macrophages in spleen, lymph nodes, thymus, liver, skin. Langerhans cells in the skin. Kupffer cells in the liver.
  • General notes
    The antigen is a 125kD cell surface membrane bound protein (non-reducing conditions). It is sensitive to 2 mercaptoethanol.

Properties

Applications

Our Abpromise guarantee covers the use of ab15694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 25185444
Flow Cyt Use at an assay dependent concentration. ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • Tissue specificity
    Expression is restricted to eosinophils.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

Anti-F4/80 antibody [BM8] (Biotin) images

  • ab15694 staining pan Macrophage in murine liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 2% milk for 10 minutes at 20°C followed by incubation with the primary antibody at a 1/4 dilution for 2 hours at 4°C.

    See Abreview

References for Anti-F4/80 antibody [BM8] (Biotin) (ab15694)

This product has been referenced in:
  • Duan T  et al. Dietary restriction protects against diethylnitrosamine-induced hepatocellular tumorigenesis by restoring the disturbed gene expression profile. Sci Rep 7:43745 (2017). Read more (PubMed: 28262799) »
  • Malek Mohammadi M  et al. The transcription factor GATA4 promotes myocardial regeneration in neonatal mice. EMBO Mol Med 9:265-279 (2017). Read more (PubMed: 28053183) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6.0
Permeabilization
No
Blocking step
Milk as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Mr. Brendan Toohey

Verified customer

Submitted Nov 05 2010

Thank you for your enquiry and patience. I regret to inform you that this antibody has not yet been tested for application in IHC-P (paraffin sections) and so I cannot provide you with the protocol that was used. I have removed this application from th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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