Anti-F4/80 antibody [BM8] (Biotin) (ab15694)

Overview

  • Product nameAnti-F4/80 antibody [BM8] (Biotin)
    See all F4/80 primary antibodies
  • Description
    Rat monoclonal [BM8] to F4/80 (Biotin)
  • ConjugationBiotin
  • SpecificityMouse: major subpopulation of resident tissue macrophages Although several publications have used this antibody succesfully in human, we have been unable to obtain positive results in this species and so do not guarantee it.
  • Tested applicationsSuitable for: IHC-Fr, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Unfortunately, this information is considered to be commercially sensitive

  • EpitopeF4/80 antigen.
  • Positive control
    • Macrophages in spleen, lymph nodes, thymus, liver, skin. Langerhans cells in the skin. Kupffer cells in the liver.
  • General notesThe antigen is a 125kD cell surface membrane bound protein (non-reducing conditions). It is sensitive to 2 mercaptoethanol.

Properties

Applications

Our Abpromise guarantee covers the use of ab15694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 0.5 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionOrphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • Tissue specificityExpression is restricted to eosinophils.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

Anti-F4/80 antibody [BM8] (Biotin) images

  • ab15694 staining pan Macrophage in murine liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 2% milk for 10 minutes at 20°C followed by incubation with the primary antibody at a 1/4 dilution for 2 hours at 4°C.

    See Abreview

References for Anti-F4/80 antibody [BM8] (Biotin) (ab15694)

This product has been referenced in:
  • Gerlini A  et al. The role of host and microbial factors in the pathogenesis of pneumococcal bacteraemia arising from a single bacterial cell bottleneck. PLoS Pathog 10:e1004026 (2014). Flow Cyt ; Mouse . Read more (PubMed: 24651834) »
  • Gumucio J  et al. T lymphocytes are not required for the development of fatty degeneration after rotator cuff tear. Bone Joint Res 3:262-72 (2014). IHC-Fr ; Rat . Read more (PubMed: 25185444) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6.0
Permeabilization No
Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Mr. Brendan Toohey

Verified customer

Submitted Nov 05 2010

Thank you for your enquiry and patience. I regret to inform you that this antibody has not yet been tested for application in IHC-P (paraffin sections) and so I cannot provide you with the protocol that was used. I have removed this application from th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"